Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP
文献类型：期刊论文
| 作者 | Xu, Zhaowei1; Zhang, Hainan1; Zhang, Xingrun2,3; Jiang, Hewei1; Liu, Chengxi1; Wu, Fanlin1; Qian, Lili4,5; Hao, Bingbing4,5; Czajkowsky, Daniel M.6; Guo, Shujuan1 |
| 刊名 | EMBO JOURNAL
 |
| 出版日期 | 2019-08-16 |
| 页码 | 18 |
| ISSN号 | 0261-4189 |
| 关键词 | c-di-GMP CobB diguanylate cyclase negative feedback loop protein acetylation |
| DOI | 10.15252/embj.2018100948 |
| 通讯作者 | Tao, Sheng-ce(taosc@sjtu.edu.cn) |
| 英文摘要 | As a ubiquitous bacterial secondary messenger, c-di-GMP plays key regulatory roles in processes such as bacterial motility and transcription regulation. CobB is the Sir2 family protein deacetylase that controls energy metabolism, chemotaxis, and DNA supercoiling in many bacteria. Using an Escherichia coli proteome microarray, we found that c-di-GMP strongly binds to CobB. Further, protein deacetylation assays showed that c-di-GMP inhibits the activity of CobB and thereby modulates the biogenesis of acetyl-CoA. Interestingly, we also found that one of the key enzymes directly involved in c-di-GMP production, DgcZ, is a substrate of CobB. Deacetylation of DgcZ by CobB enhances its activity and thus the production of c-di-GMP. Our work establishes a novel negative feedback loop linking c-di-GMP biogenesis and CobB-mediated protein deacetylation. |
| WOS关键词 | ESCHERICHIA-COLI ; SIRTUIN DEACETYLASE ; ACETYLATION ; MECHANISM ; PHOSPHODIESTERASE ; QUANTIFICATION ; IDENTIFICATION ; METABOLISM ; REVEALS ; DOMAIN |
| 资助项目 | National Key Research and Development Program of China[2016YFA0500600] ; National Natural Science Foundation of China[31670831] ; National Natural Science Foundation of China[31370813] ; National Natural Science Foundation of China[31370750] ; National Natural Science Foundation of China[31670722] ; National Natural Science Foundation of China[31670066] ; National Basic Research Program of China (973 Program)[2014CBA02004] ; National Science & Technology Major Project Key New Drug Creation and Manufacturing Program[2018ZX09711002-004] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000481589400001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288530]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Tao, Sheng-ce |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China 2.Tsinghua Univ, Ctr Struct Biol, Sch Life Sci, MOE Key Lab Prot Sci, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Dept Basic Med Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Chem Prote Ctr, Shanghai, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai, Peoples R China 7.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China 8.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Key Lab Noncoding RNA, Beijing, Peoples R China 9.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing, Peoples R China 10.Foshan Univ, Sch Stomatol & Med, Foshan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhaowei,Zhang, Hainan,Zhang, Xingrun,et al. Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP[J]. EMBO JOURNAL,2019:18. |
| APA | Xu, Zhaowei.,Zhang, Hainan.,Zhang, Xingrun.,Jiang, Hewei.,Liu, Chengxi.,...&Tao, Sheng-ce.(2019).Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP.EMBO JOURNAL,18. |
| MLA | Xu, Zhaowei,et al."Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP".EMBO JOURNAL (2019):18. |
入库方式： OAI收割
来源：上海药物研究所
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