Ligand-induced activation of ERK1/2 signaling by constitutively active G(s)-coupled 5-HT receptors
文献类型：期刊论文
| 作者 | Liu, Ping1,2; Yin, Yu-ling1,2; Wang, Ting1,2,3; Hou, Li1 ; Wang, Xiao-xi1; Wang, Man1; Zhao, Guan-guan1; Shi, Yi1; Xu, H. Eric1,2,3,4; Jiang, Yi1,2
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2019-09-01 |
| 卷号 | 40期号:9页码:1157-1167 |
| 关键词 | 5-HT4R 5-HT6R 5-HT7AR 5-HT ERK1/2 G(s) beta-arrestin Fyn Src |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/s41401-018-0204-6 |
| 通讯作者 | Xu, H. Eric(eric.xu@simm.ac.cn) ; Jiang, Yi(yijiang@simm.ac.cn) |
| 英文摘要 | 5-HT4R, 5-HT6R, and 5-HT7AR are three constitutively active G(s)-coupled 5-HT receptors that have key roles in brain development, learning, memory, cognition, and other physiological processes in the central nervous system. In addition to G(s) signaling cascade mediated by these three 5-HT receptors, the ERK1/2 signaling which is dependent on cyclic adenosine monophosphate (cAMP) production and protein kinase A (PKA) activation downstream of G(s) signaling has also been widely studied. In this study, we investigated these two signaling pathways originating from the three G(s)-coupled 5-HT receptors in AD293 cells. We found that the phosphorylation and activation of ERK1/2 are ligand-induced, in contrast to the constitutively active G(s) signaling. This indicates that G(s) signaling alone is not sufficient for ERK1/2 activation in these three 5-HT receptors. In addition to G(s), we found that beta-arrestin and Fyn are essential for the activation of ERK1/2. Together, these results put forth a novel mechanism for ERK1/2 activation involving the cooperative action of G(s), beta-arrestin, and Fyn. |
| WOS关键词 | G-PROTEIN ; BETA-ARRESTIN ; REGULATED KINASE ; SPLICE VARIANTS ; EXPRESSION ; PATHWAYS ; PARADIGMS ; MECHANISM ; MAPK ; SRC |
| 资助项目 | National Natural Science Foundation[31770796] ; National Science and Technology Major Project[2018ZX09711002-002-002] ; Ministry of Science and Technology of China[XDB08020303] ; K.C. Wong Education Foundation ; Youth Innovation Promotion Association of CAS ; Outstanding Young Scientist Foundation (CAS) |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000484000100003 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288832]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xu, H. Eric; Jiang, Yi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, VARI SIMM Ctr,Ctr Struct & Funct Drug Targets, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA |
| 推荐引用方式 GB/T 7714 | Liu, Ping,Yin, Yu-ling,Wang, Ting,et al. Ligand-induced activation of ERK1/2 signaling by constitutively active G(s)-coupled 5-HT receptors[J]. ACTA PHARMACOLOGICA SINICA,2019,40(9):1157-1167. |
| APA | Liu, Ping.,Yin, Yu-ling.,Wang, Ting.,Hou, Li.,Wang, Xiao-xi.,...&Jiang, Yi.(2019).Ligand-induced activation of ERK1/2 signaling by constitutively active G(s)-coupled 5-HT receptors.ACTA PHARMACOLOGICA SINICA,40(9),1157-1167. |
| MLA | Liu, Ping,et al."Ligand-induced activation of ERK1/2 signaling by constitutively active G(s)-coupled 5-HT receptors".ACTA PHARMACOLOGICA SINICA 40.9(2019):1157-1167. |
入库方式： OAI收割
来源：上海药物研究所
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