Determining the protective effects of Yin-Chen-Hao Tang against acute liver injury induced by carbon tetrachloride using 16S rRNA gene sequencing and LC/MS-based metabolomics
文献类型：期刊论文
| 作者 | Liu, Fang1; Sun, Zhaolin1; Hu, Pei1; Tian, Qiang2; Xu, Zhou1; Li, Zhixiong1 ; Tian, Xiaoting1; Chen, Mingcang1; Huang, Chenggang1
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 出版日期 | 2019-09-10 |
| 卷号 | 174页码:567-577 |
| ISSN号 | 0731-7085 |
| 关键词 | Yin-Chen-Hao Tang Liver injury Metabolomics Gut microbiota LC-MS Gene sequencing |
| DOI | 10.1016/j.jpba.2019.06.028 |
| 通讯作者 | Chen, Mingcang(baidulyac@126.com) ; Huang, Chenggang(cghsimm@126.com) |
| 英文摘要 | Yin-Chen-Hao Tang (YCHT), consisting of Artemisia annua L, Gardenia jasminoides Ellis, and Rheum Palmatum L, has been used to relieve liver diseases in China for thousands of years. Several protective mechanisms of YCHT on liver injury have been investigated based on metabolomics, but the effects of YCHT on the alterations in the gut microbiota are still unclear. In this study, an integrated approach based on 16S rRNA gene sequencing combined with high-performance liquid chromatography-mass spectrometry (HPLC-MS) metabolic profiling was performed to assess the effects of YCHT on liver injury induced by carbon tetrachloride (CCl4) through the regulation of the relative abundances of gut microbiota and their relationships with biomarker candidates. A total of twelve significantly altered bacterial genera and nine metabolites were identified, which returned to normal levels after YCHT treatment. The relative abundances of the identified microbiota, including significantly elevated amounts of p_Firmicutes, c_Clostridia, o_Clostridiales, f_Ruminococcaceae, g-[Eubacterium]_coprostanoligenes_group, s_uncultured_bacterium_f_Lachnospiraceae and remarkedly increased amounts of p_Bacteroidetes, c_Bacteroidia, o_Bacteroidales, f_Bacteroidaceae, g_Bacteroides and s_uncultured_bacterium_g_Bacteroides, were found in model rats compared with controls. Potential biomarkers, including lower levels of LysoPC (16:1(9Z)/0:0), LysoPC (20:3(5Z,8Z,11Z)), LysoPC (17:0), LysoPC (20:1(11Z)) and 3-hydroxybutyric acid and higher amounts of ornithine, L-kynurenine, hippuric acid and taurocholic acid are involved in several custom metabolic pathways, such as arginine and proline metabolism, tryptophan metabolism, glycerophospholipid metabolism and primary bile acid biosynthesis. Interestingly, there was a strong correlation between the perturbed gut microbiota in genera c_Clostridia and o_Clostridiales and the altered plasma metabolite 3-hydroxybutyric acid. This finding means that the hepatoprotective effects of YCHT may be due to the regulation of the production of the functional metabolite 3-hydroxybutyric acid through changes in the proportions of c_Clostridia and o_Clostridiales. These results showed that the hepatoprotective effects of YCHT not only focused on custom metabolic pathways but also depended on the changes in the gut microbiota in liver injury. These findings suggest that the 16S rRNA gene sequencing and LC-MS based metabolomics approach can be applied to comprehensively evaluate the effects of traditional Chinese medicines (TCMs). (C) 2019 Elsevier B.V. All rights reserved. |
| WOS关键词 | ACUTE HEPATIC-INJURY ; PATTERN-RECOGNITION ; BILE-ACID ; METABOLISM ; FIBROSIS ; RATS |
| 资助项目 | National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09731016-003] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09201001-001-008] ; National Natural Science Foundation of China for Young Scientists[81803828] ; China Postdoctoral Science Foundation[2018M642123] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000479328000066 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/288861]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Mingcang; Huang, Chenggang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Zhangjiang Hitech Pk,501 Haike Rd, Shanghai 201203, Peoples R China 2.Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang 621010, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fang,Sun, Zhaolin,Hu, Pei,et al. Determining the protective effects of Yin-Chen-Hao Tang against acute liver injury induced by carbon tetrachloride using 16S rRNA gene sequencing and LC/MS-based metabolomics[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2019,174:567-577. |
| APA | Liu, Fang.,Sun, Zhaolin.,Hu, Pei.,Tian, Qiang.,Xu, Zhou.,...&Huang, Chenggang.(2019).Determining the protective effects of Yin-Chen-Hao Tang against acute liver injury induced by carbon tetrachloride using 16S rRNA gene sequencing and LC/MS-based metabolomics.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,174,567-577. |
| MLA | Liu, Fang,et al."Determining the protective effects of Yin-Chen-Hao Tang against acute liver injury induced by carbon tetrachloride using 16S rRNA gene sequencing and LC/MS-based metabolomics".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 174(2019):567-577. |
入库方式： OAI收割
来源：上海药物研究所
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