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High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination

文献类型：期刊论文

作者Lin, Ping1,2; Pu, Qinqin2; Wu, Qun2,3; Zhou, Chuanmin2; Wang, Biao2; Schettler, Jacob2; Wang, Zhihan2; Qin, Shugang2; Gao, Pan2; Li, Rongpeng4
刊名NATURE COMMUNICATIONS
出版日期2019-08-19
卷号10页码:12
ISSN号2041-1723
DOI10.1038/s41467-019-11695-8
通讯作者Lan, Lefu(llan@simm.ac.cn) ; Jiang, Jianxin(hellojjx@126.com) ; Wu, Min(min.wu@und.edu)
英文摘要Discovery of CRISPR-Cas systems is one of paramount importance in the field of microbiology. Currently, how CRISPR-Cas systems are finely regulated remains to be defined. Here we use small regulatory RNA (sRNA) library to screen sRNAs targeting type I-F CRISPR-Cas system through proximity ligation by T4 RNA ligase and find 34 sRNAs linking to CRISPR loci. Among 34 sRNAs for potential regulators of CRISPR, sRNA pant463 and PhrS enhance CRISPR loci transcription, while pant391 represses their transcription. We identify PhrS as a regulator of CRISPR-Cas by binding CRISPR leaders to suppress Rho-dependent transcription termination. PhrS-mediated anti-termination facilitates CRISPR locus transcription to generate CRISPR RNA (crRNA) and subsequently promotes CRISPR-Cas adaptive immunity against bacteriophage invasion. Furthermore, this also exists in type I-C/-E CRISPR-Cas, suggesting general regulatory mechanisms in bacteria kingdom. Our findings identify sRNAs as important regulators of CRISPR-Cas, extending roles of sRNAs in controlling bacterial physiology by promoting CRISPR-Cas adaptation priming.
WOS关键词QUORUM SENSING CONTROLS ; TRANSCRIPTION TERMINATION ; PSEUDOMONAS-AERUGINOSA ; ADAPTIVE IMMUNITY ; BACTERIA ; BIOLOGY
资助项目National Institutes of Health[AI101973-01] ; National Institutes of Health[R01AI109317-01A1] ; National Institutes of Health[R01AI0138203-01] ; Key Program of National Nature Science Foundation of China[81530063]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000481606500008
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.183/handle/2S10ELR8/289014]  
专题中国科学院上海药物研究所
通讯作者Lan, Lefu; Jiang, Jianxin; Wu, Min
作者单位1.Army Med Univ, Daping Hosp, Inst Surg Res, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China
2.Univ North Dakota, Sch Med & Hlth Sci, Dept Biomed Sci, Grand Forks, ND USA
3.Shanghai Jiao Tong Univ, Sch Med Shanghai, Ruijin Hosp, Dept Pediat, Shanghai, Peoples R China
4.Jiangsu Normal Univ, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou, Jiangsu, Peoples R China
5.Southwestern Med Univ, Pulm & Allergy Inst, Affiliated Hosp, Luzhou, Peoples R China
6.Dalhousie Univ, Dept Microbiol & Immunol, Halifax, NS, Canada
7.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Lin, Ping,Pu, Qinqin,Wu, Qun,et al. High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination[J]. NATURE COMMUNICATIONS,2019,10:12.
APA Lin, Ping.,Pu, Qinqin.,Wu, Qun.,Zhou, Chuanmin.,Wang, Biao.,...&Wu, Min.(2019).High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination.NATURE COMMUNICATIONS,10,12.
MLA Lin, Ping,et al."High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination".NATURE COMMUNICATIONS 10(2019):12.

入库方式： OAI收割

来源：上海药物研究所

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