Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal
文献类型：期刊论文
| 作者 | Guo, Liu-bin1; Yu, Chuan2,3; Ling, Qing-lan2,3; Fu, Yu2,3; Wang, Yu-jun2,3 ; Liu, Jing-gen2,3
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| 刊名 | BEHAVIOURAL BRAIN RESEARCH
 |
| 出版日期 | 2019-10-17 |
| 卷号 | 372页码:8 |
| 关键词 | Morphine withdrawal Aversive memories Proteomics Synaptic plasticity Amygdala Dorsal hippocampus |
| ISSN号 | 0166-4328 |
| DOI | 10.1016/j.bbr.2019.112008 |
| 通讯作者 | Liu, Jing-gen(jgliu@mail.shcnc.ac.cn) |
| 英文摘要 | Addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations, encoded by changes of gene expression. We previously demonstrated that the changes in synaptic plasticity were required for the formation of aversive memories associated with morphine withdrawal. However, the proteins involved in synaptic plasticity and aversive memory formation have not been well explored. In the present study, we employed a two-dimensional gel electrophoresis (2-DE)-based proteomic technique to detect the changes of protein expression in the nucleus accumbens, amygdala and dorsal hippo campus of the rats that had developed conditioned morphine withdrawal. We found that twenty-three proteins were significantly altered in the amygdala and dorsal hippocampus after conditioned morphine withdrawal. These proteins can be classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and protein metabolism according to their biological functions. Eight proteins related to synaptic plasticity were further confirmed by western blot analysis. It is very likely that these identified proteins may contribute to conditioned morphine withdrawal-induced neural plasticity and aversive memory formation. Thus, our work will help understand the potential mechanism associated with generation of drug withdrawal memories. |
| WOS关键词 | ACTIN REARRANGEMENTS ; TWINFILIN ISOFORMS ; OPIOID RECEPTOR ; DRUG-WITHDRAWAL ; NMDA RECEPTORS ; EXPRESSION ; MEMORY ; CALCINEURIN ; PLASTICITY ; ADDICTION |
| 资助项目 | National Natural Science Foundation of China[81671322] |
| WOS研究方向 | Behavioral Sciences ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000478709300011 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289021]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Jing-gen |
| 作者单位 | 1.China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 210009, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Collaborat Innovat Ctr Brain Sci, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Liu-bin,Yu, Chuan,Ling, Qing-lan,et al. Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal[J]. BEHAVIOURAL BRAIN RESEARCH,2019,372:8. |
| APA | Guo, Liu-bin,Yu, Chuan,Ling, Qing-lan,Fu, Yu,Wang, Yu-jun,&Liu, Jing-gen.(2019).Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal.BEHAVIOURAL BRAIN RESEARCH,372,8. |
| MLA | Guo, Liu-bin,et al."Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal".BEHAVIOURAL BRAIN RESEARCH 372(2019):8. |
入库方式： OAI收割
来源：上海药物研究所
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