Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity
文献类型：期刊论文
| 作者 | Zhang, Shihai1,2; He, Fei1,2; Chen, Xia1,2; Ding, Kan1,2
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2019-11-01 |
| 卷号 | 223页码:10 |
| ISSN号 | 0144-8617 |
| 关键词 | Rhamnogalacturonan I (RG-I) Polysaccharide Lycium ruthenicum Murr Pancreatic cancer |
| DOI | 10.1016/j.carbpol.2019.115104 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE Sepharose (TM) Fast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked beta-D-Galp, 1,3-linked beta-D-Galp, 1,6-linked beta-D-Galp, 1,3,6-linked beta-D-Galp, 1,5-linked alpha-L-Araf, 1,3,5-linked alpha-L-Araf, T-linked alpha-L-Araf and T-linked beta-D-Xylp. Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3 beta and p-p38 MAP kinase. |
| WOS关键词 | ANTIOXIDANT ACTIVITY ; POLYSACCHARIDE ; CANCER ; FRUIT ; ARABINOGALACTAN ; ANGIOGENESIS ; ELUCIDATION ; METASTASIS ; FLAVONOIDS ; FEATURES |
| 资助项目 | National Natural Science Foundation of China[31870801] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020373] ; Program of Shanghai Subject Chief Scientist[16XD1404500] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000481570700042 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289028]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Kan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Materia Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shihai,He, Fei,Chen, Xia,et al. Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity[J]. CARBOHYDRATE POLYMERS,2019,223:10. |
| APA | Zhang, Shihai,He, Fei,Chen, Xia,&Ding, Kan.(2019).Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity.CARBOHYDRATE POLYMERS,223,10. |
| MLA | Zhang, Shihai,et al."Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity".CARBOHYDRATE POLYMERS 223(2019):10. |
入库方式： OAI收割
来源：上海药物研究所
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