Magnesium lithospermate B ameliorates microcirculation perfusion in rats by promoting vascular NO production via activating the PI3K/AKT pathway
文献类型：期刊论文
| 作者 | Liu, Ying-luo1,2; Zhou, Xiao-yu1; Xuan, Li-jiang2,3
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| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2019-08-01 |
| 卷号 | 40期号:8页码:1010-1018 |
| ISSN号 | 1671-4083 |
| 关键词 | traditional Chinese medicine Salvia miltiorrhiza magnesium lithospermate B microcirculation dysfunction NO endothelium nitric oxide synthase PI3K/AKT pathway |
| DOI | 10.1038/s41401-018-0203-7 |
| 通讯作者 | Xuan, Li-jiang(ljxuan@simm.ac.cn) |
| 英文摘要 | Microcirculation morphologically refers to the blood flow in vessels of less than 150 mu m in diameter, including arterioles, capillaries and venules, which provides nutrients and removes metabolic byproducts within tissues. Microcirculation dysfunction is involved in the pathological progress of many diseases, such as obesity, hypertension, and insulin resistance. In this study we investigated the effects of magnesium lithospermate B (MLB), an active compound of the traditional Chinese medicine Slavia miltiorrhiza, on the microcirculation dysfunction in rats and the underlying molecular mechanisms. The effects of MLB on microcirculation were assessed in vivo by measuring the hindlimb blood perfusion in dextran-induced microcirculation dysfunction rats and mesentery blood flow in anesthetized rats. We demonstrated that administration of MLB restored the impaired rat hindlimb blood flow and promoted the mesenteric micoperfusion in vivo. We further revealed in these two animal models that MLB treatment significantly increased the production of total nitrite in vascular tissues (mesentery, aorta, and heart), which was confirmed in human microvascular endothelial cells (HMEC-1) treated with MLB in vitro. Moreover, we showed that MLB treatment significantly increased the phosphorylation of endothelium nitric oxide synthase (eNOS) via inducing AKT phosphorylation in vivo and in vitro. Co-administration of the eNOS inhibitor L-NAME (20 mg/kg) abolished the protective effects of MLB against dextran-induced microcirculation dysfunction in rats, whereas pretreatment with PI3K inhibitor LY294002 (10 mu M) prevented eNOS activation in MLB-treated HMEC-1 cells. Our results suggest that MLB can restore the microcirculation dysfunction via activating eNOS, and in turn enhancing the vascular nitric oxide production, which is medicated by MLB-caused activation of the PI3K/AKT pathway. |
| WOS关键词 | NITRIC-OXIDE ; MICROVASCULAR DYSFUNCTION ; ISCHEMIA/REPERFUSION INJURY ; ENDOTHELIUM |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000477938200003 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289090]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xuan, Li-jiang |
| 作者单位 | 1.Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Shanghai Inst Mat Med, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying-luo,Zhou, Xiao-yu,Xuan, Li-jiang. Magnesium lithospermate B ameliorates microcirculation perfusion in rats by promoting vascular NO production via activating the PI3K/AKT pathway[J]. ACTA PHARMACOLOGICA SINICA,2019,40(8):1010-1018. |
| APA | Liu, Ying-luo,Zhou, Xiao-yu,&Xuan, Li-jiang.(2019).Magnesium lithospermate B ameliorates microcirculation perfusion in rats by promoting vascular NO production via activating the PI3K/AKT pathway.ACTA PHARMACOLOGICA SINICA,40(8),1010-1018. |
| MLA | Liu, Ying-luo,et al."Magnesium lithospermate B ameliorates microcirculation perfusion in rats by promoting vascular NO production via activating the PI3K/AKT pathway".ACTA PHARMACOLOGICA SINICA 40.8(2019):1010-1018. |
入库方式： OAI收割
来源：上海药物研究所
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