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Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction

文献类型：期刊论文

作者Li, Bai1; Zhou, Xian-Hao2,3,4; Yang, Peng-Yu1; Zhu, Liping1; Zhong, Yuan1; Cai, Zhengjun1; Jiang, Biao3,4; Cai, Xiaoqing1; Liu, Jia3; Jiang, Xianxing1
刊名ADVANCED SCIENCE
出版日期2019-07-03
卷号6期号:13页码:11
关键词fluorogenic labeling nitroso-diene cycloaddition photoactivatable protein imaging
DOI10.1002/advs.201802039
通讯作者Liu, Jia(liujia@shanghaitech.edu.cn) ; Jiang, Xianxing(jiangxx5@mail.sysu.edu.cn)
英文摘要Fluorogenic labeling enables imaging cellular molecules of interest with minimal background. This process is accompanied with the notable increase of the quantum yield of fluorophore, thus minimizing the background signals from unactivated profluorophores. Herein, the development of a highly efficient and bioorthogonal nitroso-based Diels-Alder fluorogenic reaction is presented and its usefulness is validated as effective and controllable in fluorescent probes and live-cell labeling strategies for dynamic cellular imaging. It is demonstrated that nitroso-based cycloaddition is an efficient fluorogenic labeling tool through experiments of further UV-activatable fluorescent labeling on proteins and live cells. The ability of tuning the fluorescence of labeled proteins by UV-irradiation enables selective activation of proteins of interest in a particular cell compartment at a given time point, while leaving the remaining labeled molecules untouched.
WOS关键词INDEPENDENT CHEMICAL-SHIFTS ; LIVE-CELL ; TETRAZINE PROBES ; IN-VIVO ; LIGATION ; CYCLOADDITION ; FLUORESCENCE ; TECHNOLOGY ; CHEMISTRY
资助项目Major Research Program of National Natural Science Foundation of China[91853106] ; Program for Guangdong Introducing Innovative and Enterpreneurial Teams[2016ZT06Y337] ; ShanghaiTech University[2019F0301-000-01] ; Thousand Young Talents Program
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000477710600006
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/289100]  
专题中国科学院上海药物研究所
通讯作者Liu, Jia; Jiang, Xianxing
作者单位1.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China
3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Li, Bai,Zhou, Xian-Hao,Yang, Peng-Yu,et al. Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction[J]. ADVANCED SCIENCE,2019,6(13):11.
APA Li, Bai.,Zhou, Xian-Hao.,Yang, Peng-Yu.,Zhu, Liping.,Zhong, Yuan.,...&Jiang, Xianxing.(2019).Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction.ADVANCED SCIENCE,6(13),11.
MLA Li, Bai,et al."Photoactivatable Fluorogenic Labeling via Turn-On "Click-Like" Nitroso-Diene Bioorthogonal Reaction".ADVANCED SCIENCE 6.13(2019):11.

入库方式： OAI收割

来源：上海药物研究所

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