Hepatoprotective effects of a traditional Chinese medicine formula against carbon tetrachloride-induced hepatotoxicity in vivo and in vitro
文献类型：期刊论文
| 作者 | Ren, Xia1,4; Xin, Lan-Ting1,4; Zhang, Meng-Qi1,4; Zhao, Qing1,4; Yue, Shi-Yun1,4; Chen, Kai-Xian2,3 ; Guo, Yue-Wei2,3; Shao, Chang-Lun1,4; Wang, Chang-Yun1,4
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| 刊名 | BIOMEDICINE & PHARMACOTHERAPY
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| 出版日期 | 2019-09-01 |
| 卷号 | 117页码:8 |
| 关键词 | Traditional Chinese medicine Le-Cao-Shi formula Acute liver injury Hepatoprotective effect |
| ISSN号 | 0753-3322 |
| DOI | 10.1016/j.biopha.2019.109190 |
| 通讯作者 | Shao, Chang-Lun() ; Wang, Chang-Yun(changyun@ouc.edu.cn) |
| 英文摘要 | Le-Cao-Shi (LCS), a formula of Traditional Chinese Medicine (TCM), has been used as a folk medicine for protection and treatment of liver injury. However, scientific evidences on its hepatoprotective effects have not been investigated. In this study, hepatoprotective activities of LCS water extracts (LCS-W) and ethanol extracts (LCS-E) against carbon tetrachloride (CCl4)-induced liver damage were investigated in vivo and in vitro. In vivo experiments, pretreatment of LCS-W and LCS-E to rats significantly declined the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and markedly increased the activity of superoxide dismutase (SOD) and ameliorated the level of malondialdehyde (MDA) induced by CCl4 treatment. Especially, LCS-WM group significantly prevented the elevation of lipid peroxidation level induced by CCl4, with the MDA level closed to that of normal group. Histopathological examinations further confirmed that LCS-W and LCS-E could protect the liver cells from CCl4-induced damage. In addition, immunohistochemically analysis revealed that LCS-W could significantly down-regulated the hepatic protein expression of necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta). Correspondingly, LCS-W and LCS-E were observed to promote cell viability and decline the levels of ALT, AST, and lactate dehydrogenase (LDH) in vitro. It could be concluded that LCS can exert a protective effect against CCl4-induced hepatotoxicity, which might be a potential therapeutic prescription for preventing or treating liver injury. Notably, LCS-W displayed better hepatoprotective activity against CCl4-induced injury than that of LCS-E, suggesting that LCS extracted by water decoction has good development prospects. Our results contribute towards the validation of the traditional use of LCS in the treatment of liver disorders. |
| WOS关键词 | ACUTE LIVER-INJURY ; ANTIOXIDANT ACTIVITY ; EXTRACT ; DAMAGE ; POLYSACCHARIDES ; RATS |
| 资助项目 | National Natural Science Fund of China[41806191] ; National Natural Science Fund of China[U1606403] ; National High Technology Research and Development Program of China (863 Program)[2013AA093001] ; Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0406-5] ; Taishan Scholars Program, China |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000477804500046 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289218]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Shao, Chang-Lun; Wang, Chang-Yun |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Open Studio Druggabil Res Marine Nat Prod, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Ocean Univ China, Sch Med & Pharm, Minist Educ China, Key Lab Marine Drugs, 5 Yushan Rd, Qingdao 266237, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Xia,Xin, Lan-Ting,Zhang, Meng-Qi,et al. Hepatoprotective effects of a traditional Chinese medicine formula against carbon tetrachloride-induced hepatotoxicity in vivo and in vitro[J]. BIOMEDICINE & PHARMACOTHERAPY,2019,117:8. |
| APA | Ren, Xia.,Xin, Lan-Ting.,Zhang, Meng-Qi.,Zhao, Qing.,Yue, Shi-Yun.,...&Wang, Chang-Yun.(2019).Hepatoprotective effects of a traditional Chinese medicine formula against carbon tetrachloride-induced hepatotoxicity in vivo and in vitro.BIOMEDICINE & PHARMACOTHERAPY,117,8. |
| MLA | Ren, Xia,et al."Hepatoprotective effects of a traditional Chinese medicine formula against carbon tetrachloride-induced hepatotoxicity in vivo and in vitro".BIOMEDICINE & PHARMACOTHERAPY 117(2019):8. |
入库方式： OAI收割
来源：上海药物研究所
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