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Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system

文献类型：期刊论文

作者Qiu, Wenqing1,2; Xu, Zhijiao1,2; Zhang, Min3; Zhang, Dandan1,2; Fan, Hui4,5; Li, Taotao1,2; Wang, Qianfeng4,5; Liu, Peiru1,2; Zhu, Zaihua6; Du, Duo1,2
刊名NUCLEIC ACIDS RESEARCH
出版日期2019-05-21
卷号47期号:9页码:14
ISSN号0305-1048
DOI10.1093/nar/gkz134
通讯作者Liu, Yun(yliu39@fudan.edu.cn)
英文摘要The architecture and function of chromatin are largely regulated by local interacting molecules, such as transcription factors and noncoding RNAs. However, our understanding of these regulatory molecules at a given locus is limited because of technical difficulties. Here, we describe the use of Clustered Regularly Interspaced Short Palindromic Repeats and an engineered ascorbate peroxidase 2 (APEX2) system to investigate local chromatin interactions (CAPLOCUS). We showed that with specific small-guide RNA targets, CAPLOCUS could efficiently identify both repetitive genomic regions and single-copy genomic locus with high resolution. Genome-wide sequencing revealed known and potential long-range chromatin interactions for a specific single-copy locus. CAPLOCUS also identified telomere-associated RNAs. CAPLOCUS, followed by mass spectrometry, identified both known and novel telomere-associated proteins in their native states. Thus, CAPLOCUS may be a useful approach for studying local interacting molecules at any given chromosomal location.
WOS关键词TELOMERE LENGTH ; DNA ELEMENTS ; RNA TERRA ; GENOME ; PROTEINS ; REVEALS ; BINDING ; ORGANIZATION ; IDENTIFICATION ; PRINCIPLES
资助项目National Natural Science Foundation of China[31771451] ; National Natural Science Foundation of China[31471212] ; National Natural Science Foundation of China[31670066] ; National Key R&D Program of China[2017YFC0909200] ; National Basic Research Program of China (973 program)[2014CBA02004]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000473756300004
出版者OXFORD UNIV PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/289312]  
专题新药研究国家重点实验室
通讯作者Liu, Yun
作者单位1.Fudan Univ, Zhongshan Hosp, MOE Key Lab Metab & Mol Med, Dept Biochem & Mol Biol,Sch Basic Med Sci, Shanghai 200032, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Materia Med, Chem Prote Ctr, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Materia Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Fudan Univ, Sch Basic Med Sci, Inst Biomed Sci, MOE Key Lab Metab & Mol Med, Shanghai 200032, Peoples R China
5.Fudan Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Shanghai 200032, Peoples R China
6.Fudan Univ, Huashan Hosp, Div Rheumatol, Shanghai 200040, Peoples R China
推荐引用方式
GB/T 7714		 Qiu, Wenqing,Xu, Zhijiao,Zhang, Min,et al. Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system[J]. NUCLEIC ACIDS RESEARCH,2019,47(9):14.
APA Qiu, Wenqing.,Xu, Zhijiao.,Zhang, Min.,Zhang, Dandan.,Fan, Hui.,...&Liu, Yun.(2019).Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system.NUCLEIC ACIDS RESEARCH,47(9),14.
MLA Qiu, Wenqing,et al."Determination of local chromatin interactions using a combined CRISPR and peroxidase APEX2 system".NUCLEIC ACIDS RESEARCH 47.9(2019):14.

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来源：上海药物研究所

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