Evaluation of the chemical consistency of Yin-Chen-Hao-Tang prepared by combined and separated decoction methods using high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry coupled with multivariate statistical analysis
文献类型：期刊论文
| 作者 | Tian, Qiang1; Liu, Fang2; Xu, Zhou2; Liu, Huan2; Yin, Hao2; Sun, Zhaolin2; Chen, Mingcang2; Li, Zhixiong2 ; Ma, Lin1; Huang, Chenggang2
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2019-05-01 |
| 卷号 | 42期号:9页码:1664-1675 |
| ISSN号 | 1615-9306 |
| 关键词 | chemical consistency metabolomics quadrupole time-of-flight mass spectrometry Traditional Chinese medicines |
| DOI | 10.1002/jssc.201800961 |
| 通讯作者 | Ma, Lin(malin@swust.edu.cn) ; Huang, Chenggang(cghsimm@126.com) |
| 英文摘要 | In this study, Yin-Chen-Hao-Tang prepared by two decoction methods, namely, combined decoction (modern decoction method) and separated decoction (traditional decoction method), was analyzed by high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. The acquired datasets containing sample codes, tR-m/z pairs and ion intensities were processed with multivariate statistical analyses, such as principal component analysis and an orthogonal partial least squared discriminant analysis model, to globally compare the chemical differences between the different decoction samples. Then, the chemical differences between the combined and separated decoctions were screened out by S-plots generated from the orthogonal partial least squared discriminant analysis model and compared with chemical information from an established in-house library. The six components that contributed the most to the chemical differences were identified as chlorogenic acid, caffeic acid, geniposide, genipin, scopoletin, and 3,5-dicaffeoylquinic acid. The concentrations of genipin and caffeic acid from the separated decoction were higher than those from the combined decoction, indicating that the separated decoction may present a stronger hepatoprotective effect. However, the results still require further investigation through pharmacological and clinical studies. Our findings not only establish a strategy to evaluate chemical consistency of Yin-Chen-Hao-Tang but also provide the scientific basis for using traditional separated decoction method. |
| WOS关键词 | CHINESE MEDICINE FORMULA ; LIVER-INJURY ; RAT PLASMA ; DIFFERENTIATION ; CONSTITUENTS ; PROTEOMICS ; PROTECTS ; GINSENG ; ROOTS |
| 资助项目 | National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09731016-003] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09201001-001-008] ; National Natural Science Foundation of China for Young Scientists[81803828] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000471322300017 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289385]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ma, Lin; Huang, Chenggang |
| 作者单位 | 1.Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang, Sichuan, Peoples R China 2.Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Qiang,Liu, Fang,Xu, Zhou,et al. Evaluation of the chemical consistency of Yin-Chen-Hao-Tang prepared by combined and separated decoction methods using high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry coupled with multivariate statistical analysis[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(9):1664-1675. |
| APA | Tian, Qiang.,Liu, Fang.,Xu, Zhou.,Liu, Huan.,Yin, Hao.,...&Huang, Chenggang.(2019).Evaluation of the chemical consistency of Yin-Chen-Hao-Tang prepared by combined and separated decoction methods using high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry coupled with multivariate statistical analysis.JOURNAL OF SEPARATION SCIENCE,42(9),1664-1675. |
| MLA | Tian, Qiang,et al."Evaluation of the chemical consistency of Yin-Chen-Hao-Tang prepared by combined and separated decoction methods using high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry coupled with multivariate statistical analysis".JOURNAL OF SEPARATION SCIENCE 42.9(2019):1664-1675. |
入库方式： OAI收割
来源：上海药物研究所
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