lncRNA H19 binds VGF and promotes pNEN progression via PI3K/AKT/CREB signaling
文献类型：期刊论文
| 作者 | Ji, Meng1; Yao, Yanli2,3; Liu, Anan1; Shi, Ligang1; Chen, Danlei1; Tang, Liang1; Yang, Guang1; Liang, Xing1; Peng, Junfeng1; Shao, Chenghao1 |
| 刊名 | ENDOCRINE-RELATED CANCER
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 26期号:7页码:643-658 |
| ISSN号 | 1351-0088 |
| 关键词 | H19 VGF long non-coding RNA pancreatic neuroendocrine neoplasm PI3K/AKT/CREB |
| DOI | 10.1530/ERC-18-0552 |
| 通讯作者 | Shao, Chenghao(shaochenghao_czyy@163.com) |
| 英文摘要 | Pancreatic neuroendocrine neoplasms (pNENs) are endocrine tumors arising in pancreas and is the most common neuroendocrine tumors. Mounting evidence indicates IncRNA H19 could be a determinant of tumor progression. However, the expression and mechanism of H19 and the relevant genes mediated by H19 in pNENs remain undefined. Microarray analysis was conducted to identify the differentially expressed lncRNAs in pNENs. H19 expression was analyzed in 39 paired pNEN tissues by qPCR. The biological role of H19 was determined by functional experiments. RNA pulldown, mass spectroscopy and RNA immunoprecipitation were performed to confirm the interaction between H19 and VGF. RNA-seq assays were performed after knockdown H19 or VGF. H19 was significantly upregulated in pNEN tissues with malignant behaviors, and the upregulation predicted poor prognosis in pNENs. In vitro and in vivo data showed that H19 overexpression promoted tumor growth and metastasis, whereas H19 knockdown led to the opposite phenotypes. H19 interacted with VGF, which was significantly upregulated in pNENs, and higher VGF expression was markedly related to poor differentiation and advanced stage. Furthermore, VGF was downregulated when H19 was knocked down, and VGF promoted cell proliferation, migration and invasion. Mechanistic investigations revealed that H19 activated PI3K/AKT/CREB signaling and promoted pNEN progression by interacting with VGF. These findings indicate that H19 is a promising prognostic factor in pNENs with malignant behaviors and functions as an oncogene via the VGF-mediated PI3K/AKT/CREB pathway. In addition, our study implies that VGF may also serve as a candidate prognostic biomarker and therapeutic target in pNENs. |
| WOS关键词 | PANCREATIC NEUROENDOCRINE TUMORS ; LONG NONCODING RNA ; CHROMOGRANIN-A ; EXPRESSION ; PROLIFERATION ; METASTASIS ; SURVIVAL ; GROWTH ; CELLS ; MICE |
| 资助项目 | Medical Guidance Project of Shanghai Science and Technology Commission[124119a0501] |
| WOS研究方向 | Oncology ; Endocrinology & Metabolism |
| 语种 | 英语 |
| 出版者 | BIOSCIENTIFICA LTD |
| WOS记录号 | WOS:000472611400003 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289404]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Shao, Chenghao |
| 作者单位 | 1.Second Mil Med Univ, Shanghai Changzheng Hosp, Dept Pancreat Biliary Surg, Dept Gen Surg, Shanghai, Peoples R China 2.Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, Shanghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Meng,Yao, Yanli,Liu, Anan,et al. lncRNA H19 binds VGF and promotes pNEN progression via PI3K/AKT/CREB signaling[J]. ENDOCRINE-RELATED CANCER,2019,26(7):643-658. |
| APA | Ji, Meng.,Yao, Yanli.,Liu, Anan.,Shi, Ligang.,Chen, Danlei.,...&Shao, Chenghao.(2019).lncRNA H19 binds VGF and promotes pNEN progression via PI3K/AKT/CREB signaling.ENDOCRINE-RELATED CANCER,26(7),643-658. |
| MLA | Ji, Meng,et al."lncRNA H19 binds VGF and promotes pNEN progression via PI3K/AKT/CREB signaling".ENDOCRINE-RELATED CANCER 26.7(2019):643-658. |
入库方式： OAI收割
来源：上海药物研究所
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