Application of multiple chemical and biological approaches for quality assessment of Carthamus tinctorius L. (safflower) by determining both the primary and secondary metabolites
文献类型：期刊论文
| 作者 | Lu, Jia-Xi1; Zhanga, Chun-Xia1,2; Hu, Ying1,2; Zhang, Meng-Han1; Wang, Ya-Nan3,4; Qian, Yue-Xin1,2; Yang, Jing1; Yang, Wen-Zhi1,2; Jiang, Miao-Miao1,2; Guo, De-An1,5
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| 刊名 | PHYTOMEDICINE
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| 出版日期 | 2019-05-01 |
| 卷号 | 58页码:12 |
| 关键词 | UHPLC/Q-Orbitrap MS Quantitative H-1 NMR Carthamus tinctorius Primary metabolite Secondary metabolite Quality evaluation |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2019.152826 |
| 通讯作者 | Yang, Wen-Zhi(ywz_0504@163.com) ; Jiang, Miao-Miao(miaomiaojiang@tjutcm.edu.cn) ; Guo, De-An(gda5958@163.com) |
| 英文摘要 | Background: The florets of Carthamus tinctorius L. (safflower) serve as the source of a reputable herbal medicine targeting gynecological diseases. Conventional investigations regarding the quality control of safflower, however, mainly focused on the secondary metabolites with primary metabolites ignored. Purpose: To holistically evaluate the quality difference of safflower samples collected from five different producing regions by multiple chemical and biological approaches with both the primary and secondary metabolites considered. Methods: A precursor ions list-triggered data-dependent MS2 approach was established by ultra-high performance liquid chromatography/Q-Orbitrap mass spectrometry (UHPLC/Q-Orbitrap MS) to comprehensively identify the secondary metabolites from safflower. Primary metabolites were identified by various 1D and 2D nuclear magnetic resonance (NMR) experiments. Similarity evaluation and quantitative assays of all the characterized primary metabolites and a quinochalcone C-glycoside (QCG) marker, hydroxysafflor yellow A (HSYA), were performed by quantitative H-1 NMR (qNMR) using an external standard method. Multiple in vitro models with respect to the antioxidant, anti-platelet aggregation, and antioxidant stress injury effects, were assayed to determine the efficacy differences. Results: Totally thirteen primary metabolites (including one nucleoside, two sugars, five organic alkali/acids, and five amino acids) and 135 secondary metabolites (97 QCGs and 38 flavonoids) could be identified or tentatively characterized from safflower. Good chemical consistency was observed between the commercial safflower samples and a standard safflower sample, with similarity varying in the range of 0.95-0.99. The results from qNMR-oriented quantitative experiments (thirteen primary metabolites and HSYA) and biological assays indicated the quality of safflower samples from Xinjiang (XJ-2 and XJ-4), Hunan (HuN-1 and HuN-2), and Sichuan (SC), was comparable to the standard safflower sample. Conclusion: The integration of multiple chemical (using two analytical platforms, UHPLC/Q-Orbitrap MS and NMR) and biological (four in vitro models) approaches by determining both the primary and secondary metabolites demonstrated a powerful strategy that could facilitate the holistic quality evaluation of traditional Chinese medicine. |
| WOS关键词 | QUINOCHALCONE C-GLYCOSIDES ; MASS-SPECTROMETRY ANALYSIS ; CHROMATOGRAPHY ; IDENTIFICATION ; DISCOVERY ; STRATEGY ; MARKERS ; FLORETS |
| 资助项目 | National Natural Science Foundation of China[81573547] ; National Natural Science Foundation of China[81503239] ; National Natural Science Foundation of China[81872996] ; State Project for Essential Drug Research and Development[2015ZX09501004-003-004] ; State Project for Essential Drug Research and Development[2017ZX09101003-009-010] ; State Key Research and Development Project-Special Research of TCM Modernization[2017YFC1702104] ; Tianjin Municipal Education Commission Research Project[2017ZD07] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000473047100017 |
| 出版者 | ELSEVIER GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289461]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Wen-Zhi; Jiang, Miao-Miao; Guo, De-An |
| 作者单位 | 1.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, 312 Anshanxi Rd, Tianjin 300193, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, 312 Anshanxi Rd, Tianjin 300193, Peoples R China 3.Chinese Acad Med Sci, Inst Mat Med, Beijing, Peoples R China 4.Peking Union Med Coll, Beijing, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Jia-Xi,Zhanga, Chun-Xia,Hu, Ying,et al. Application of multiple chemical and biological approaches for quality assessment of Carthamus tinctorius L. (safflower) by determining both the primary and secondary metabolites[J]. PHYTOMEDICINE,2019,58:12. |
| APA | Lu, Jia-Xi.,Zhanga, Chun-Xia.,Hu, Ying.,Zhang, Meng-Han.,Wang, Ya-Nan.,...&Guo, De-An.(2019).Application of multiple chemical and biological approaches for quality assessment of Carthamus tinctorius L. (safflower) by determining both the primary and secondary metabolites.PHYTOMEDICINE,58,12. |
| MLA | Lu, Jia-Xi,et al."Application of multiple chemical and biological approaches for quality assessment of Carthamus tinctorius L. (safflower) by determining both the primary and secondary metabolites".PHYTOMEDICINE 58(2019):12. |
入库方式： OAI收割
来源：上海药物研究所
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