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An AIE-based self-assembled fluorescent probe for COX-2 imaging

文献类型：期刊论文

作者Cao, Xiaozheng1; Gao, Tang1; Dong, Jie1,2; Jiang, Xinchen1; Zou, Hui3; Liu, Tingting4; Yu, Kundian4; Zeng, Wenbin1
刊名NEW JOURNAL OF CHEMISTRY
出版日期2019-05-28
卷号43期号:20页码:7874-7880
ISSN号1144-0546
DOI10.1039/c9nj01473j
通讯作者Zeng, Wenbin(wbzeng@hotmail.com)
英文摘要Cyclooxygenase-2 (COX-2), an isozyme of the cyclooxygenase enzyme family, is not only highly expressed in various types of tumors, but also over-expressed in all stages of cancer. Herein, we report the design and preparation of an aggregation-induced emission (AIE) based fluorescent probe TPI-IMC for the imaging of COX-2 by the conjugation of 4-(1,4,5-triphenyl-1H-imidazol-2-yl)pyridine (TPI) and COX-2 inhibitor indomethacin (IMC). The probe was applied for the detection and imaging of COX-2, and a linear range from 0 to 0.4 g mL(-1) and a detection limit of 0.08 g mL(-1)in vitro were achieved. TPI-IMC exhibits weak fluorescence in normal cells (LO2 cells and RAW264.7 cells), while it exhibits stronger fluorescence in the cells over-expressing COX-2 such as cancer cells (HeLa cells) and inflammatory cells (LPS induced RAW264.7 cells). It is also found that significantly reduced fluorescence intensity is observed and detected in HeLa cells co-treated with the COX-2 inhibitor celecoxib. Therefore, TPI-IMC has the potential to be used as a guiding-agent in surgery for tumours and inflammation.
WOS关键词CANCER BIOMARKERS ; CYCLOOXYGENASE-2 ; STATISTICS ; EXPRESSION ; CELLS ; TUMOR
资助项目National Natural Science Foundation of China[81741134] ; National Natural Science Foundation of China[81671756] ; Key Research Project of Science and Technology Foundation of Hunan Province[2017SK2093] ; State Key Laboratory of Drug Research[SIMM1803KF-14] ; Key Research Project of Science and Technology Foundation of Changsha[kq1801063] ; Fundamental Research Funds for the Central Universities of Central South University[2017zzts222] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts041]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000472216200038
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.183/handle/2S10ELR8/289499]  
专题新药研究国家重点实验室
通讯作者Zeng, Wenbin
作者单位1.Cent S Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China
2.Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Coll Food Sci & Engn, Changsha, Hunan, Peoples R China
3.Hunan Normal Univ, Med Coll, Changsha, Hunan, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Cao, Xiaozheng,Gao, Tang,Dong, Jie,et al. An AIE-based self-assembled fluorescent probe for COX-2 imaging[J]. NEW JOURNAL OF CHEMISTRY,2019,43(20):7874-7880.
APA Cao, Xiaozheng.,Gao, Tang.,Dong, Jie.,Jiang, Xinchen.,Zou, Hui.,...&Zeng, Wenbin.(2019).An AIE-based self-assembled fluorescent probe for COX-2 imaging.NEW JOURNAL OF CHEMISTRY,43(20),7874-7880.
MLA Cao, Xiaozheng,et al."An AIE-based self-assembled fluorescent probe for COX-2 imaging".NEW JOURNAL OF CHEMISTRY 43.20(2019):7874-7880.

入库方式： OAI收割

来源：上海药物研究所

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