Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 1: Metabolic profiles from the liver-centric perspective
文献类型：期刊论文
| 作者 | Liu, Huan1,2; Chen, Mingcang1,2; Yin, Hao1,2; Hu, Pei1,2; Wang, Yangyang1,2; Liu, Fang1,2; Tian, Xiaoting1,2; Huang, Chenggang1,2
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2019-06-12 |
| 卷号 | 237页码:81-91 |
| 关键词 | Yin-chen-hao tang Liver Metabolites Liver injury HPLC-Q-TOF Carbon tetrachloride |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2019.03.044 |
| 通讯作者 | Tian, Xiaoting(zhghydxtxt@163.com) ; Huang, Chenggang(cghsimm@126.com) |
| 英文摘要 | Ethnopharmacological relevance: Yin-Chen-Hao Tang (YCHT), derived from "Treatise on Febrile Diseases" in ancient China, has been a very popular hepatoprotective three-herb formula in China and Japan, although its chemical base remains unclear. Aim of this study: As the first step in revealing the hepatoprotective chemical base of YCHT, we aimed to clarify the absorbed ingredients and associated metabolic pathways for orally dosed YCHT in both normal and liver injury rats from a liver-centric perspective. Materials and methods: With the aid of 10 reference compounds, the absorbed ingredients and generated metabolites were systematically characterized by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF) in the portal vein plasma (the plasma before hepatic disposition) - liver - systemic plasma (the plasma after hepatic disposition), following oral administration of YCHT in normal and CCl4-induced liver injury rats. Results: A total of 38 compounds with six chemical structures, consisting of 10 prototypes and 28 metabolites generated through 9 biotransformations, were absolutely or tentatively identified, and 25 compounds were first reported on YCHT treatments. Among them, 8 compounds were absolutely confirmed by comparing with standard substances, and some had published hepatoprotective activities. Compared with the 35, 15, and 29 compounds identified in the portal vein plasma, liver, and systemic plasma of normal rats, respectively, the corresponding numbers of characterized compounds were 37, 13 and 29 in the liver injury rats. Conclusions: Sulfation and glucuronidation were the predominant biotransformations, and intestinal metabolism, prior to hepatic metabolism, occurred for most compounds. CCl4-induced liver injury caused only slight changes in the metabolic profiles of rats orally administered YCHT. These results provided the precondition for further quantitative analysis and pharmacodynamic screening of compounds in YCHT. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ALPHA-NAPHTHYLISOTHIOCYANATE ; YINCHENHAO DECOCTION ; HEPATIC-FAILURE ; IN-VITRO ; MEDICINE ; PHARMACOKINETICS ; HEPATOTOXICITY ; ANTHRAQUINONES ; CONSTITUENTS |
| 资助项目 | National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09731016-003] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018ZX09201001-001-008] ; Shanghai Science and Technology Development Funds[18401931300] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2019280] ; National Natural Science Foundation for Young Scientists of China[81603280] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000469152400010 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289699]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Tian, Xiaoting; Huang, Chenggang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Hai Ke Rd Zhangjiang, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Huan,Chen, Mingcang,Yin, Hao,et al. Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 1: Metabolic profiles from the liver-centric perspective[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,237:81-91. |
| APA | Liu, Huan.,Chen, Mingcang.,Yin, Hao.,Hu, Pei.,Wang, Yangyang.,...&Huang, Chenggang.(2019).Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 1: Metabolic profiles from the liver-centric perspective.JOURNAL OF ETHNOPHARMACOLOGY,237,81-91. |
| MLA | Liu, Huan,et al."Exploration of the hepatoprotective chemical base of an orally administered herbal formulation (YCHT) in normal and CCl4-intoxicated liver injury rats. Part 1: Metabolic profiles from the liver-centric perspective".JOURNAL OF ETHNOPHARMACOLOGY 237(2019):81-91. |
入库方式： OAI收割
来源：上海药物研究所
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