中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
The Protective Effect of Magnesium Lithospermate B on Hepatic Ischemia/Reperfusion via Inhibiting the Jak2/Stat3 Signaling Pathway

文献类型：期刊论文

作者Zhang, Ning1; Han, Li2,3; Xue, Yaru2,3; Deng, Qiangqiang2; Wu, Zhitao2; Peng, Huige2; Zhang, Yiting2,3; Xuan, Lijiang2,3; Pan, Guoyu2,3; Fu, Qiang1
刊名FRONTIERS IN PHARMACOLOGY
出版日期2019-05-31
卷号10页码:13
关键词magnesium lithospermate B hepatic ischemia/reperfusion acute injury anti-inflammation Jak2/Stat3 signaling pathway
ISSN号1663-9812
DOI10.3389/fphar.2019.00620
通讯作者Pan, Guoyu(gypan@simm.ac.cn) ; Fu, Qiang(744298001@qq.com)
英文摘要Acute inflammation is an important component of the pathogenesis of hepatic ischemia/reperfusion injury (HIRI). Magnesium lithospermate B (MLB) has strong neuroprotective and cardioprotective effects. The purpose of this study was to determine whether MLB had underlying protective effects against hepatic I/R injury and to reveal the potential mechanisms related to the hepatoprotective effects. In this study, we first examined the protective effect of MLB on HIRI in mice that underwent 1 h ischemia followed by 6 h reperfusion. MLB pretreatment alleviated the abnormal liver function and hepatocyte damage induced by I/R injury. We found that serum inflammatory cytokines, including IL-6, IL-1 beta, and TNF-alpha, were significantly decreased by MLB during hepatic ischemia/reperfusion (I/R) injury, suggesting that MLB may alleviate hepatic I/R injury via inhibiting inflammatory signaling pathways. Second, we investigated the protein level of p-Jak2/Jak2 and p-Stat3/Stat3 using Western blotting and found that MLB could significantly inhibit the activation of the Jak2/Stat3 signaling pathway, which was further verified by AG490 in a mouse model. Finally, the effect of MLB on the Jak2/Stat3 pathway was further assessed in an in vitro model of RAW 264.7 cells; 1 mu g/ml LPS induced the secretion of inflammatory mediators, including IL-6, TNF-alpha, and activation of the Jak2/Stat3 signaling pathway. MLB significantly inhibited the abnormal secretion of inflammatory factors and the activation of the Jak2/Stat3 signaling pathway in RAW264.7 cells. In conclusion, MLB was found for the first time to reduce inflammation induced by hepatic I/R via suppressing the Jak2/Stat3 pathway.
WOS关键词NF-KAPPA-B ; LIVER ISCHEMIA ; REPERFUSION INJURY ; CEREBRAL-ISCHEMIA ; JAK/STAT PATHWAY ; JANUS KINASE-2 ; INFLAMMATION ; APOPTOSIS ; CELLS ; TRANSPLANTATION
资助项目Independent Deployment Program of the Institute of Pharmaceutical Innovation of the Chinese Academy of Sciences[CASIMM0120184005] ; National Science Foundation of China[81872927] ; Organ Reconstruction and Manufacturing Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020205]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000470145600001
出版者FRONTIERS MEDIA SA
源URL[http://119.78.100.183/handle/2S10ELR8/289741]  
专题中国科学院上海药物研究所
通讯作者Pan, Guoyu; Fu, Qiang
作者单位1.China Pharmaceut Univ, Dept Pharmacol Chinese Mat Med, Nanjing, Jiangsu, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Ning,Han, Li,Xue, Yaru,et al. The Protective Effect of Magnesium Lithospermate B on Hepatic Ischemia/Reperfusion via Inhibiting the Jak2/Stat3 Signaling Pathway[J]. FRONTIERS IN PHARMACOLOGY,2019,10:13.
APA Zhang, Ning.,Han, Li.,Xue, Yaru.,Deng, Qiangqiang.,Wu, Zhitao.,...&Fu, Qiang.(2019).The Protective Effect of Magnesium Lithospermate B on Hepatic Ischemia/Reperfusion via Inhibiting the Jak2/Stat3 Signaling Pathway.FRONTIERS IN PHARMACOLOGY,10,13.
MLA Zhang, Ning,et al."The Protective Effect of Magnesium Lithospermate B on Hepatic Ischemia/Reperfusion via Inhibiting the Jak2/Stat3 Signaling Pathway".FRONTIERS IN PHARMACOLOGY 10(2019):13.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。