Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains
文献类型：期刊论文
| 作者 | Zhang, Shihai1,2,4; Zhang, Chunqian2,3,4; Li, Meixia2,4; Chen, Xia2,4; Ding, Kan1,2,4
|
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 128页码:435-443 |
| 关键词 | Crataegus pinnatifida Glucan Human gut microbiota Bacteroides |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2019.01.158 |
| 通讯作者 | Chen, Xia(xiachen@simm.accn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | Crataegus pinnanfida is widely used as functional food and traditional medicine in China for various applications. Polysaccharides may contribute to health benefits, such as modulating human gut microbiota (HGM). To investigate structure features of polysaccharides from this plant, fruits of C. pinnanfida were extracted with boiling water, yielding crude polysaccharides HAW. A novel polysaccharide, named HAW1-1, was pooled by DEAE Sepharosen (TM) Fast Flow column and further purified by Sephacryl S-100 FIR column. The molecular weight of HAW1-1 was 42.35 kDa. The carbohydrate content of HAW1-1 was 99.42% and no protein was detected. The monosaccharide analysis showed this polysaccharide was only composed of glucose. According to the partial acid hydrolysis, methylation analysis and NMR spectra, the backbone of HAW1-1 was consisted of 1,4-linked alpha-D-glucan and 1,4,6-linked alpha-D-glucan. The side chains were composed of 1,6-linked alpha-D-glucan and terminal-linked alpha-D-glucan. The bioactivity tests showed that HAW1-1 could promote the growth of three species of intestinal Bacteroides and produce short-chain fatty acids (SCFAs). The results suggested that HAW1-1 might be a potential prebiotic candidate to maintain intestinal homeostasis and improve human gut health. (C) 2019 Published by Elsevier B.V. |
| WOS关键词 | CHAIN FATTY-ACIDS ; ANTIOXIDANT ACTIVITY ; CHINESE HAWTHORN ; FRUIT ; POLYSACCHARIDE ; METABOLISM ; BUTYRATE ; FIBER |
| 资助项目 | New Drug Creation and Manufacturing Program[2017ZX09305-003] ; Ministry of Science and Technology of the People's Republic of China ; National Natural Science Foundation of China[31670814] ; Program of Shanghai Subject Chief Scientist[16XD1404500] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000463305100052 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/289986]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Xia; Ding, Kan |
| 作者单位 | 1.Zunyi Med Univ, Sch Pharm, 201 Dalian Rd, Zunyi 563003, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Shanghai Univ, Sch Life Sci, 99 Shangda Rd, Shanghai 200444, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Olcochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shihai,Zhang, Chunqian,Li, Meixia,et al. Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,128:435-443. |
| APA | Zhang, Shihai,Zhang, Chunqian,Li, Meixia,Chen, Xia,&Ding, Kan.(2019).Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,128,435-443. |
| MLA | Zhang, Shihai,et al."Structural elucidation of a glucan from Crataegus pinnatifida and its bioactivity on intestinal bacteria strains".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 128(2019):435-443. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。