Temperature- and rigidity-mediated rapid transport of lipid nanovesicles in hydrogels
文献类型：期刊论文
| 作者 | Yu, Miaorong1,2; Song, Wenyi1; Tian, Falin3; Dai, Zhuo1; Zhu, Quanlei1; Ahmad, Ejaj1; Guo, Shiyan1; Zhu, Chunliu1 ; Zhong, Haijun4; Yuan, Yongchun5
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| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2019-03-19 |
| 卷号 | 116期号:12页码:5362-5369 |
| ISSN号 | 0027-8424 |
| 关键词 | lipid nanovesicle liposome phase transition temperature diffusion biological hydrogels |
| DOI | 10.1073/pnas.1818924116 |
| 通讯作者 | Shi, Xinghua(shixh@nanoctr.cn) ; Gan, Yong(ygan@simm.ac.cn) ; Gao, Huajian(huajian_gao@brown.edu) |
| 英文摘要 | Lipid nanovesicles are widely present as transport vehicles in living organisms and can serve as efficient drug delivery vectors. It is known that the size and surface charge of nanovesicles can affect their diffusion behaviors in biological hydrogels such as mucus. However, how temperature effects, including those of both ambient temperature and phase transition temperature (T-m), influence vehicle transport across various biological barriers outside and inside the cell remains unclear. Here, we utilize a series of liposomes with different T-m as typical models of nanovesicles to examine their diffusion behavior in vitro in biological hydrogels. We observe that the liposomes gain optimal diffusivity when their T-m is around the ambient temperature, which signals a drastic change in the nanovesicle rigidity, and that liposomes with T-m around body temperature (i.e., similar to 37 degrees C) exhibit enhanced cellular uptake in mucus-secreting epithelium and show significant improvement in oral insulin delivery efficacy in diabetic rats compared with those with higher or lower T-m. Molecular-dynamics (MD) simulations and superresolution microscopy reveal a temperature- and rigidity-mediated rapid transport mechanism in which the liposomes frequently deform into an ellipsoidal shape near the phase transition temperature during diffusion in biological hydrogels. These findings enhance our understanding of the effect of temperature and rigidity on extracellular and intracellular functions of nanovesicles such as endosomes, exosomes, and argosomes, and suggest that matching T-m to ambient temperature could be a feasible way to design highly efficient nanovesicle-based drug delivery vectors. |
| WOS关键词 | EXTRACELLULAR VESICLES ; GASTROINTESTINAL MUCUS ; MECHANICAL-PROPERTIES ; BENDING RIGIDITY ; DRUG-DELIVERY ; ORAL DELIVERY ; NANOPARTICLES ; PHASE ; TRANSLOCATION ; FORMULATIONS |
| 资助项目 | National Natural Science Foundation of China[81573378] ; National Natural Science Foundation of China[81773651] ; National Natural Science Foundation of China[11422215] ; National Natural Science Foundation of China[11272327] ; National Natural Science Foundation of China[11672079] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA01020304] ; Shanghai Sailing Program 2017[17YF1423500] ; K. C. Wong Education Foundation ; Opening Fund of State Key Laboratory of Nonlinear Mechanics ; New Star Program, Shanghai Institute of Materia Medica, Chinese Academy of Sciences ; Super-computing Center of Chinese Academy of Sciences ; [CASIMM0120153020] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000461679000030 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/290171]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Shi, Xinghua; Gan, Yong; Gao, Huajian |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Key Lab Nanosyst & Hierarchy Fabricat, Lab Theoret & Computat Nanosci, Ctr Excellence Nanosci,Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China 4.Nanchang Univ, Coll Med, Dept Pharm, Nanchang 330066, Jiangxi, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Tech Phys, Shanghai 200083, Peoples R China 6.Peking Univ, Beijing Innovat Ctr Engn Sci & Adv Technol, Coll Engn, Dept Mech & Engn Sci, Beijing 100871, Peoples R China 7.Brown Univ, Sch Engn, Providence, RI 02912 USA |
| 推荐引用方式 GB/T 7714 | Yu, Miaorong,Song, Wenyi,Tian, Falin,et al. Temperature- and rigidity-mediated rapid transport of lipid nanovesicles in hydrogels[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(12):5362-5369. |
| APA | Yu, Miaorong.,Song, Wenyi.,Tian, Falin.,Dai, Zhuo.,Zhu, Quanlei.,...&Gao, Huajian.(2019).Temperature- and rigidity-mediated rapid transport of lipid nanovesicles in hydrogels.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(12),5362-5369. |
| MLA | Yu, Miaorong,et al."Temperature- and rigidity-mediated rapid transport of lipid nanovesicles in hydrogels".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.12(2019):5362-5369. |
入库方式： OAI收割
来源：上海药物研究所
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