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Multi-omic measurements of heterogeneity in HeLa cells across laboratories

文献类型：期刊论文

作者Liu, Yansheng1,2; Mi, Yang3,4; Mueller, Torsten5; Kreibich, Saskia6; Williams, Evan G.5; Van Drogen, Audrey5; Borel, Christelle7,8; Franks, Max5; Germain, Pierre-Luc9; Bludau, Isabell5
刊名NATURE BIOTECHNOLOGY
出版日期2019-03-01
卷号37期号:3页码:314-+
ISSN号1087-0156
DOI10.1038/s41587-019-0037-y
通讯作者Liu, Yansheng(yansheng.liu@yale.edu) ; Aebersold, Ruedi(aebersold@imsb.biol.ethz.ch)
英文摘要Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14 stock HeLa samples from 13 international laboratories. We cultured cells in uniform conditions and profiled genome-wide copy numbers, mRNAs, proteins and protein turnover rates in each cell line. We discovered substantial heterogeneity between HeLa variants, especially between lines of the CCL2 and Kyoto varieties, and observed progressive divergence within a specific cell line over 50 successive passages. Genomic variability has a complex, nonlinear effect on transcriptome, proteome and protein turnover profiles, and proteotype patterns explain the varying phenotypic response of different cell lines to Salmonella infection. These findings have implications for the interpretation and reproducibility of research results obtained from human cultured cells.
WOS关键词PROTEIN ; DYNAMICS ; QUANTIFICATION ; AUTHENTICATION ; TURNOVER ; RESOURCE ; DATABASE ; PEPTIDE ; LINES
资助项目SystemsX. ch project PhosphoNetX PPM ; TargetInfectX ; Swiss National Science Foundation[3100A0-688 107679] ; Swiss National Science Foundation[163180] ; European Research Council[ERC-20140AdG 670821] ; European Research Council[AdG 249968] ; European Research Council[616441-DISEASEAVATARS] ; JRC for Computational Biomedicine ; Bayer AG ; Umberto Veronesi Foundation ; ERA-NET Neuron Program ; Regione Lombardia (Ricerca Indipendente 2012) ; Italian Ministry of Health (Ricerca Corrente) ; NIH F32 Ruth Kirchstein Fellowship[F32GM119190]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000460155900023
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.183/handle/2S10ELR8/290338]  
专题中国科学院上海药物研究所
通讯作者Liu, Yansheng; Aebersold, Ruedi
作者单位1.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA
2.Yale Univ, Yale Canc Biol Inst, West Haven, CT 06516 USA
3.Heidelberg Univ, Fac Biosci, Heidelberg, Germany
4.Rhein Westfal TH Aachen, Fac Med, Joint Res Ctr Computat Biomed JRC COMBINE, Aachen, Germany
5.Swiss Fed Inst Technol, Dept Biol, Inst Mol Syst Biol, Zurich, Switzerland
6.Swiss Fed Inst Technol, Inst Microbiol, Zurich, Switzerland
7.Univ Geneva, Dept Genet Med & Dev, Med Sch, Geneva, Switzerland
8.Univ Hosp Geneva, Geneva, Switzerland
9.European Inst Oncol, IEO, IRCCS, Milan, Italy
10.Tech Univ Dresden, Carl Gustav Carus Fac Med, Inst Med Informat & Biometry, Dresden, Germany
推荐引用方式
GB/T 7714		 Liu, Yansheng,Mi, Yang,Mueller, Torsten,et al. Multi-omic measurements of heterogeneity in HeLa cells across laboratories[J]. NATURE BIOTECHNOLOGY,2019,37(3):314-+.
APA Liu, Yansheng.,Mi, Yang.,Mueller, Torsten.,Kreibich, Saskia.,Williams, Evan G..,...&Aebersold, Ruedi.(2019).Multi-omic measurements of heterogeneity in HeLa cells across laboratories.NATURE BIOTECHNOLOGY,37(3),314-+.
MLA Liu, Yansheng,et al."Multi-omic measurements of heterogeneity in HeLa cells across laboratories".NATURE BIOTECHNOLOGY 37.3(2019):314-+.

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来源：上海药物研究所

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