Earth's transition from an icehouse to hothouse during the Late Paleozoic was characterized by a series of high paleolatitude glacial-interglacial events. However, the exact timing of these events remains unresolved. Here, we report on fifty-five calcitic shells of brachiopods and bivalves screened to obtain reliable 87Sr/86Sr ratios for Cisuralian (298.9-272.95 Ma) and Guadalupian (272.95-259.1 Ma) seawater. Specifically, we used well-preserved shells to build a 87Sr/86Sr seawater curve for the Sydney Basin, and then with the marine Look-up Table of the Permian calculated numerical ages of the stratigraphic succession. This allowed us to match seawater changes to the coeval P1 - P3 glacial-interglacial cycles recorded in the sedimentary successions of the Sydney Basin. Evaluation of the 87Sr/86Sr results revealed that onsets of the P2 and P3 glaciations in the Sydney Basin should be assigned to the early Artinskian and late Wordian, respectively. The range of 87Sr/86Sr values recorded by brachiopods from the Wandrawandian Siltstone coupled with recent geochronological dating of the Broughton Formation suggest that glacial phase P3 lasted about 2 Myr and was confined to the late Wordian - early Capitanian. Dating obtained using the Sr-isotope proxy from brachiopods agrees with the geochronologic ages, and they suggest that the glacial phases P1 to P3 became progressively shorter in duration and less intense. Conversely, the corresponding interglacials became progressively longer, and thus, documenting the gradual transition of the Permian icehouse to an ice-free greenhouse world. Our study confirms that strontium isotopes measured in screened brachiopod shell archives are sufficiently robust to date Paleozoic marine sedimentary successions, and are most valuable in sedimentary successions that otherwise lack geo-chronological dating. (C) 2019 Elsevier B.V. All rights reserved.