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Packaging and delivering enzymes by amorphous metal-organic frameworks

文献类型：期刊论文

作者Wu, Xiaoling3; Yue, Hua4; Zhang, Yuanyu2; Gao, Xiaoyong4; Li, Xiaoyang2; Wang, Licheng2; Cao, Yufei2; Hou, Miao2; An, Haixia4; Zhang, Lin5,6
刊名NATURE COMMUNICATIONS
出版日期2019-11-14
卷号10页码:8
ISSN号2041-1723
DOI10.1038/s41467-019-13153-x
英文摘要Enzymatic catalysis in living cells enables the in-situ detection of cellular metabolites in single cells, which could contribute to early diagnosis of diseases. In this study, enzyme is packaged in amorphous metal-organic frameworks (MOFs) via a one-pot co-precipitation process under ambient conditions, exhibiting 5-20 times higher apparent activity than when the enzyme is encapsulated in corresponding crystalline MOFs. Molecular simulation and cryo-electron tomography (Cryo-ET) combined with other techniques demonstrate that the mesopores generated in this disordered and fuzzy structure endow the packaged enzyme with high enzyme activity. The highly active glucose oxidase delivered by the amorphous MOF nanoparticles allows the noninvasive and facile measurement of glucose in single living cells, which can be used to distinguish between cancerous and normal cells.
WOS关键词X-RAY ; CANCER ; AMORPHIZATION ; PLATFORM
资助项目National Key Research and Development Plan of China[2016YFA0204300] ; National Key Research and Development Plan of China[2017YFA0403801] ; National Natural Science Foundation of China[21622603] ; National Natural Science Foundation of China[21622608] ; National Natural Science Foundation of China[21878174] ; National Natural Science Foundation of China[51573085] ; National Natural Science Foundation of China[21908070] ; National Natural Science Foundation of China[U1732120] ; Beijing Natural Science Foundation[JQ18006] ; China Postdoctoral Science Foundation[BX20180102]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000496409800013
出版者NATURE PUBLISHING GROUP
资助机构National Key Research and Development Plan of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; China Postdoctoral Science Foundation
源URL[http://ir.ipe.ac.cn/handle/122111/39160]  
专题中国科学院过程工程研究所
通讯作者Zhang, Lin; Li, Sai; Wei, Wei; Ge, Jun
作者单位1.Beijing Adv Innovat Ctr Struct Biol, Beijing 100084, Peoples R China
2.Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing 100084, Peoples R China
3.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
4.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
5.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Dept Biochem Engn, Tianjin 300354, Peoples R China
6.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300354, Peoples R China
7.Tsinghua Univ, Sch Life Sci, Minist Educ, Key Lab Prot Sci, Beijing 100084, Peoples R China
8.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China
9.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Zhangjiang Lab, Shanghai 201204, Peoples R China
10.Jilin Univ, State Key Lab Superhard Mat, Changchun 130012, Jilin, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Xiaoling,Yue, Hua,Zhang, Yuanyu,et al. Packaging and delivering enzymes by amorphous metal-organic frameworks[J]. NATURE COMMUNICATIONS,2019,10:8.
APA Wu, Xiaoling.,Yue, Hua.,Zhang, Yuanyu.,Gao, Xiaoyong.,Li, Xiaoyang.,...&Ge, Jun.(2019).Packaging and delivering enzymes by amorphous metal-organic frameworks.NATURE COMMUNICATIONS,10,8.
MLA Wu, Xiaoling,et al."Packaging and delivering enzymes by amorphous metal-organic frameworks".NATURE COMMUNICATIONS 10(2019):8.

入库方式： OAI收割

来源：过程工程研究所

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