Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus
文献类型：期刊论文
| 作者 | An, Ning2; Gong, Pisheng2; Hou, Henglei2; Chi, Weiya2; Jin, Haibo2; Zhao, Lan1; Tan, Qiqi2; Tang, Xu2; Wang, Fei2; Jin, Hongchao2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2020-01-11 |
| 卷号 | 1610页码:4 |
| ISSN号 | 0021-9673 |
| 关键词 | Negative chromatography Macroporous microsphere with core-shell structure Purification of virus Avian influenza virus |
| DOI | 10.1016/j.chroma.2019.460578 |
| 英文摘要 | The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700). (C) 2019 Elsevier B.V. All rights reserved. |
| WOS关键词 | PROTEIN |
| 资助项目 | Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality[IDHT20180508] ; Beijing Natural Science Foundation[2162013] ; Beijing Natural Science Foundation[2172054] ; Construction of Scientific Research Platform[2018XK002] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000509816200042 |
| 资助机构 | Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality ; Beijing Natural Science Foundation ; Construction of Scientific Research Platform |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/39285]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Rongyue |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Inst Petrochem Technol, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Coll Chem Engn, Beijing 102617, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Ning,Gong, Pisheng,Hou, Henglei,et al. Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1610:4. |
| APA | An, Ning.,Gong, Pisheng.,Hou, Henglei.,Chi, Weiya.,Jin, Haibo.,...&Zhang, Rongyue.(2020).Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus.JOURNAL OF CHROMATOGRAPHY A,1610,4. |
| MLA | An, Ning,et al."Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus".JOURNAL OF CHROMATOGRAPHY A 1610(2020):4. |
入库方式： OAI收割
来源：过程工程研究所
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