Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms
文献类型：期刊论文
| 作者 | Liu, Jia-Ning1,2; Chang, Sen-Lin1,2; Xu, Peng-Wei1,2; Tan, Ming-Hui1,2; Zhao, Bing1,2; Wang, Xiao-Dong1,2; Zhao, Qing-Sheng1,2
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| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 出版日期 | 2020-01-29 |
| 卷号 | 68期号:4页码:1101-1109 |
| ISSN号 | 0021-8561 |
| 关键词 | epsilon-poly-L-lysine conformation beta-sheet pH responsivity carbamoylated epsilon-PL antibacterial activity |
| DOI | 10.1021/acs.jafc.9b07524 |
| 英文摘要 | epsilon-Poly-L-lysine (epsilon-PL) consists of 25-35 lysine residues which are linked by an isopeptide bond formed by dehydration condensation of alpha-carboxyl and epsilon-amino groups and has good antibacterial activity and broad-spectrum inhibition range. However, there is no clear conclusion about the structure and antibacterial mechanism of epsilon-PL in aqueous solution. Herein, a high purity of epsilon-PL was prepared using Amberlite IRC-50 ion-exchange resin. Membrane filtration and dynamic light scattering were used to study the variations of epsilon-PL aggregation in aqueous solution with pH value. The conformational changes and antibacterial activities of epsilon-PL and carbamoylated epsilon-PL in different water environments were studied with circular dichroism (CD) and inhibition zone. The structural changes during the spray-drying process were determined by Fourier transform infrared spectroscopy. The results indicated that the side chain amino charge played a decisive role in the epsilon-PL conformation and aggregation. epsilon-PL exhibited the properties of a beta-sheet during spray drying from acidic liquids to solids. The cation enhanced the antibacterial activity of epsilon-PL but did not play a key role. Instead, the backbone of epsilon-PL might determine the mechanism of epsilon-PL antibacterial. |
| WOS关键词 | ANTIMICROBIAL MECHANISM ; ESCHERICHIA-COLI ; ALPHA-HELIX ; BETA-SHEET ; POLYLYSINE ; STREPTOMYCES ; POLY(EPSILON-L-LYSINE) |
| 资助项目 | Jiangsu Yiming Biological Co., Ltd. |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000510529800018 |
| 资助机构 | Jiangsu Yiming Biological Co., Ltd. |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/39362]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhao, Qing-Sheng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia-Ning,Chang, Sen-Lin,Xu, Peng-Wei,et al. Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020,68(4):1101-1109. |
| APA | Liu, Jia-Ning.,Chang, Sen-Lin.,Xu, Peng-Wei.,Tan, Ming-Hui.,Zhao, Bing.,...&Zhao, Qing-Sheng.(2020).Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68(4),1101-1109. |
| MLA | Liu, Jia-Ning,et al."Structural Changes and Antibacterial Activity of Epsilon-poly-L-lysine in Response to pH and Phase Transition and Their Mechanisms".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68.4(2020):1101-1109. |
入库方式： OAI收割
来源：过程工程研究所
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