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Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system

文献类型：期刊论文

作者Fan, Yixiao1,2; Li, Yingbo1; Liu, Qingfen1
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
出版日期2020-03-02
页码13
关键词cephalexin enzymatic synthesis penicillin G acylase suspension aqueous solution system beta-lactam antibiotics
ISSN号0885-4513
DOI10.1002/bab.1903
英文摘要An efficient method for the enzymatic synthesis of cephalexin (CEX) from 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and d-phenylglycine methyl ester (PGME) using immobilized penicillin G acylase (IPGA) as catalyst in a suspension aqueous solution system was developed, where the reactant 7-ADCA and product CEX are mainly present as solid particles. The effects of key factors on the enzymatic synthesis were investigated. Results showed that continuous feeding of PGME was more efficient for the synthesis of CEX than the batch mode. Under the optimized conditions, the maximum 7-ADCA conversion ratio of 99.3% and productivity of 200 mmol/L/H were achieved, both of which are much superior to the homogeneous aqueous solution system. Besides, IPGA still retained 95.4% of its initial activity after 10 cycles of enzymatic synthesis, indicating the excellent stability of this approach. The developed approach shows great potential for the industrial production of CEX via an enzyme-based route.
WOS关键词IMMOBILIZED PENICILLIN ACYLASE ; BETA-LACTAM ANTIBIOTICS ; KINETICALLY CONTROLLED SYNTHESIS ; HIGH SUBSTRATE CONCENTRATIONS ; SOLID PEPTIDE-SYNTHESIS ; ETHYLENE-GLYCOL MEDIUM ; SITU PRODUCT REMOVAL ; INDUSTRIAL-PRODUCTION ; CATALYZED SYNTHESIS ; ORGANIC MEDIUM
资助项目Major Science and Technology Project of Water Pollution Control and Management in China[2017ZX07402003] ; Key Research Program of the Chinese Academy of Sciences[ZDRW-ZS-2016-5-3] ; National Natural Science Foundation of China[21676272]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000517500000001
出版者WILEY
资助机构Major Science and Technology Project of Water Pollution Control and Management in China ; Key Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/39524]  
专题中国科学院过程工程研究所
通讯作者Li, Yingbo; Liu, Qingfen
作者单位1.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Fan, Yixiao,Li, Yingbo,Liu, Qingfen. Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2020:13.
APA Fan, Yixiao,Li, Yingbo,&Liu, Qingfen.(2020).Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,13.
MLA Fan, Yixiao,et al."Efficient enzymatic synthesis of cephalexin in suspension aqueous solution system".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2020):13.

入库方式： OAI收割

来源：过程工程研究所

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