HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol
文献类型：期刊论文
| 作者 | Zhang, Yao1,2; Yin, Shuang1,3; Zhang, Bingyang1,3; Bi, Jingxiu3; Liu, Yongdong1; Su, Zhiguo1 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2020-02-01 |
| 卷号 | 89页码:233-237 |
| 关键词 | Virus-like particle (VLP) Hepatitis B virus core antigen (HBc) Inclusion body Assembly Sodium dodecyl sulfate (SDS) 2-methyl-2, 4-pentanediol (MPD) |
| ISSN号 | 1359-5113 |
| DOI | 10.1016/j.procbio.2019.10.031 |
| 英文摘要 | Hepatitis B virus core antigen (HBc) has recently been used as carriers to develop recombinant vaccines. However, not virus-like particles (VLPs) but inactive inclusion bodies are often formed for the chimeric proteins when expressed in Escherichia coli. A novel method for in vitro assembly of chimeric HBc-MAGE3 II from inclusion bodies to VLPs was established in this study. The method utilized 2-methyl-2, 4-pentanediol (MPD), an amphipathic di-alcohol, to dissociate sodium dodecyl sulfate (SDS) from the solubilized chimeric protein to initiate VLP assembly. The HBc-MAGE3 II could assemble into VLPs only when the molar ratio of SDS/protein subunit was less than 0.14. After removing SDS/MPD by desalting and further purification, VLPs with similar morphology to the natural virus were obtained. This method could be used for preparation of other VLPs expressed as inclusion bodies. |
| WOS关键词 | SODIUM DODECYL-SULFATE ; SDS ; DETERGENTS ; PROTEINS ; REMOVAL ; PURIFICATION ; LYSOZYME ; EXCHANGE ; BINDING |
| 资助项目 | National Natural Science Foundation of China[21576267] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000517856100025 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/39582]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Liu, Yongdong; Su, Zhiguo |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA 5005, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, Yao,Yin, Shuang,Zhang, Bingyang,et al. HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol[J]. PROCESS BIOCHEMISTRY,2020,89:233-237. |
| APA | Zhang, Yao,Yin, Shuang,Zhang, Bingyang,Bi, Jingxiu,Liu, Yongdong,&Su, Zhiguo.(2020).HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol.PROCESS BIOCHEMISTRY,89,233-237. |
| MLA | Zhang, Yao,et al."HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol".PROCESS BIOCHEMISTRY 89(2020):233-237. |
入库方式： OAI收割
来源：过程工程研究所
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