A novel electro-driven immunochromatography assay based on upconversion nanoparticles for rapid pathogen detection
文献类型:期刊论文
作者 | Zhao, Yong1; Zhang, Pingping1; Wang, Jin1; Zhou, Lei2; Yang, Ruifu1 |
刊名 | BIOSENSORS & BIOELECTRONICS |
出版日期 | 2020-03-15 |
卷号 | 152页码:5 |
ISSN号 | 0956-5663 |
关键词 | Immunochromatography Upconversion Nanoparticles Electrokinetics Electroosmosis Yersinia Pestis |
DOI | 10.1016/j.bios.2020.112037 |
英文摘要 | Immunochromatography assays are simple and low-cost point-of-care diagnostics. However, the dependence on paper-based capillary forces limits improvements in speed of the assay. An electro-driven immunochromatography assay (EICA) is described that uses electroosmotic flow (EOF) to increase the driving force, as well as upconversion nanoparticles for high-sensitivity detection. EOF increased the signal intensity by 64.0% and reduced the assay time from 15 min to 5 min, and the EICA exhibited good specificity and sensitivity (1.2 x 10(3) CFU/mL) for pure cultures of Yersinia pestis EV76. It also had a strong tolerance to acid-base conditions (pH 2-12), salt solutions (NaCl <= 0.5 mol/L), viscous materials (polyethylene glycol 20,000 <= 25 mg/mL), and proteins (bovine serum albumin <= 200 mg/mL). For real soil samples, the EICA exhibited a low limit of detection (LOD) of 1.2 x 10(4) CFU/mL with good repeatability and reliability. Thus, EICA is applicable for detection of Y. pestis in soil, and should provide a rapid and sensitive tool for point-of-care diagnosis and detection. |
WOS关键词 | Lateral-flow Immunoassay ; Quantitative Detection ; Yersinia-pestis |
资助项目 | National Key Research and Development Program of China[2017YFC1601503] ; National Science and Technology Major Project[2018ZX10101003002-010] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
语种 | 英语 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
WOS记录号 | WOS:000515210600017 |
资助机构 | National Key Research and Development Program of China ; National Science and Technology Major Project |
源URL | [http://ir.ipe.ac.cn/handle/122111/39595] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Zhou, Lei; Yang, Ruifu |
作者单位 | 1.Beijing Inst Microbiol & Epidemiol, Belling Key Lab POCT Bioemergency & Clin, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China 2.Chinese Acad Sci, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Yong,Zhang, Pingping,Wang, Jin,et al. A novel electro-driven immunochromatography assay based on upconversion nanoparticles for rapid pathogen detection[J]. BIOSENSORS & BIOELECTRONICS,2020,152:5. |
APA | Zhao, Yong,Zhang, Pingping,Wang, Jin,Zhou, Lei,&Yang, Ruifu.(2020).A novel electro-driven immunochromatography assay based on upconversion nanoparticles for rapid pathogen detection.BIOSENSORS & BIOELECTRONICS,152,5. |
MLA | Zhao, Yong,et al."A novel electro-driven immunochromatography assay based on upconversion nanoparticles for rapid pathogen detection".BIOSENSORS & BIOELECTRONICS 152(2020):5. |
入库方式: OAI收割
来源:过程工程研究所
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