Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction
文献类型：期刊论文
| 作者 | Jing, Bolin3,4; Wang, Zhuo A.4; Zhang, Chen4; Deng, Quanfeng2; Wei, Jinhua4; Luo, Yong2; Zhang, Xiuli1; Li, Jianjun4; Du, Yuguang4 |
| 刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
 |
| 出版日期 | 2020-03-31 |
| 卷号 | 8页码:14 |
| ISSN号 | 2296-4185 |
| 关键词 | peristaltic microfluidic chip human gut-vessel co-culture model host-microbial interaction Lactobacillus casei enteritis injury |
| DOI | 10.3389/fbioe.2020.00272 |
| 英文摘要 | Intestinal floras influence a lot of biological functions of the organism. Although animal model are strong tools for researches on the relationship between host and microbe, a physiologically relevant in vitro human gut model was still required. Here, a novel human gut-vessel microfluidic system was established to study the host-microbial interaction. Peristaltic motion of the cells on the chip was driven by a pneumatic pump. When intestinal epithelial cells (Caco2) were co-cultured with vascular endothelial cells (HUVECs) on the peristaltic microfluidic chip, Caco2 showed normal barrier and absorption functions after 5 days cultivation, which generally took 21 days in static Transwell models. Intestinal microvilli and glycocalyx layer were seen after 4 days cultivation, and Lactobacillus casei was successfully co-cultured for a week in the intestinal cavity. A model for intestinal damage and inflammatory responses caused by E. coli was set up on this chip, which were successfully suppressed by Lactobacillus casei or antibiotic. In summary, this human gut-vessel microfluidic system showed a good potential for investigating the host-microbial interaction and the effect and mechanism of microbiome on intestinal diseases in vitro. |
| WOS关键词 | INFLAMMATORY-BOWEL-DISEASE ; DRUG PERMEABILITY ; ENDOTHELIAL-CELLS ; STEM-CELLS ; CACO-2 ; LACTOBACILLI ; MODEL ; DIFFERENTIATION ; BIFIDOBACTERIA ; BACTERIA |
| 资助项目 | National Natural Science Foundation of China[31870802] ; National Natural Science Foundation of China[31700705] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000526743400001 |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/40110]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Xiuli; Li, Jianjun; Du, Yuguang |
| 作者单位 | 1.Soochow Univ, Coll Pharmaceut Sci, Suzhou, Peoples R China 2.Dalian Univ Technol, Dept Chem Engn, Key Lab Fine Chem, Dalian, Peoples R China 3.Univ Chinese Acad Sci, Dept Chem, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jing, Bolin,Wang, Zhuo A.,Zhang, Chen,et al. Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:14. |
| APA | Jing, Bolin.,Wang, Zhuo A..,Zhang, Chen.,Deng, Quanfeng.,Wei, Jinhua.,...&Du, Yuguang.(2020).Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,14. |
| MLA | Jing, Bolin,et al."Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):14. |
入库方式： OAI收割
来源：过程工程研究所
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