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Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes

文献类型：期刊论文

作者Ji, Mingbo3; Li, Xianhui3; Omidvarkordshouli, Maryam3; Sigurdardottir, Sigyn Bjork3; Woodley, John M.3; Daugaard, Anders Egede2; Luo, Jianquan1; Pinelo, Manuel3
刊名SEPARATION AND PURIFICATION TECHNOLOGY
出版日期2020-09-15
卷号247页码:9
ISSN号1383-5866
关键词Lysozyme Polyelectrolyte deposition Electrostatic repulsion UF
DOI10.1016/j.seppur.2020.116936
英文摘要While retention in commercial ultrafiltration membranes is commonly governed by size exclusion, addition of charged polyelectrolytes to the membrane surface has been proposed as a facile and inexpensive method to modulate retention during filtration of charged compounds, such as proteins. This study demonstrates that selected combinations of common polyelectrolytes can be efficiently used to control the retention of proteins. We show how the retention of positively charged lysozyme increased from similar to 7% to similar to 50% when a negatively charged polyelectrolyte was deposited on a commercial polysulfone membrane, most probably due to a combination of associated pore narrowing effect and protein adsorption onto the membrane. By contrast, the retention decreased again (from similar to 50% to similar to 30%) upon further addition of a positively charged polyelectrolyte onto the membrane. The electrostatic repulsion between the positively charged surface and proteins prevented protein adsorption onto the membrane, while pore size was still large enough that size exclusion was limited. The retention decreased further to similar to 22% when increasing amounts of polyelectrolytes of the same charge as the protein were deposited on the membrane. In addition, variations in pH revealed that the negative effect of polyelectrolytes of the same charge as the protein on lysozyme retention was reversed when the charge density of lysozyme reached a certain level beyond which the positively charged electrolyte promoted dramatic increases of rejection. The results of this study suggest that use of polyelectrolyte-modified ultrafiltration membranes with a much larger pore size than the charged solute to be filtered could be a strategy to control retention of such solutes by minimizing the effect of size exclusion and fouling.
WOS关键词HOLLOW-FIBER MEMBRANES ; NANOFILTRATION MEMBRANE ; SURFACE MODIFICATION ; TRANSPORT ; PERFORMANCE ; SEPARATION ; DOPAMINE
资助项目Chinese Scholarship Council[201704910984] ; Villum Foundation (Villum Experiment grant)[00028005]
WOS研究方向Engineering
语种英语
出版者ELSEVIER
WOS记录号WOS:000536142200006
资助机构Chinese Scholarship Council ; Villum Foundation (Villum Experiment grant)
源URL[http://ir.ipe.ac.cn/handle/122111/40832]  
专题中国科学院过程工程研究所
通讯作者Luo, Jianquan; Pinelo, Manuel
作者单位1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Tech Univ Denmark, Danish Polymer Ctr, Dept Chem & Biochem Engn, Bldg 229, DK-2800 Lyngby, Denmark
3.Tech Univ Denmark, Proc & Syst Engn Ctr PROSYS, Dept Chem & Biochem Engn, Bldg 229, DK-2800 Lyngby, Denmark
推荐引用方式
GB/T 7714		 Ji, Mingbo,Li, Xianhui,Omidvarkordshouli, Maryam,et al. Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2020,247:9.
APA Ji, Mingbo.,Li, Xianhui.,Omidvarkordshouli, Maryam.,Sigurdardottir, Sigyn Bjork.,Woodley, John M..,...&Pinelo, Manuel.(2020).Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes.SEPARATION AND PURIFICATION TECHNOLOGY,247,9.
MLA Ji, Mingbo,et al."Charge exclusion as a strategy to control retention of small proteins in polyelectrolyte-modified ultrafiltration membranes".SEPARATION AND PURIFICATION TECHNOLOGY 247(2020):9.

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来源：过程工程研究所

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