Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method
文献类型：期刊论文
| 作者 | Wang,Yining3,4,5,6,7; Ge,Ge2,3,4,5,6,7; Mao,Rui2; Wang,Zhuo2; Sun,Yu-Zhe1; Du,Yu-Guang2; Gao,Xing-Hua3,4,5,6,7; Qi,Rui-Qun3,4,5,6,7; Chen,Hong-Duo3,4,5,6,7 |
| 刊名 | Virology Journal
 |
| 出版日期 | 2020-07-09 |
| 卷号 | 17期号:1 |
| 关键词 | HPV Detection LAMP Microfluidic Genotyping |
| DOI | 10.1186/s12985-020-01373-3 |
| 英文摘要 | AbstractBackgroundHuman papillomaviruses (HPVs), a group of non-enveloped small viruses with double-stranded circular DNA which lead to multiple skin diseases such as benign warts, are commonly seen in clinics. The current HPV detection systems aim mainly at mucosal HPVs, however, an efficient clinical approach for cutaneous HPVs detection is lacking.ObjectivesTo establish a rapid detection system for cutaneous HPVs using a colorimetric loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye in combination with microfluidic technology.MethodsL1 DNA sequences of the 30 cutaneous HPVs were chemically synthesized, and LAMP primers against L1 DNA were designed with use of an online LAMP designing tool. Isothermal amplification was performed with use of a water bath and the amplification results were inspected with the naked eye. Using PCR sequencing as a control method, the specificity and sensitivity of the new detection system were obtained by detecting clinical samples.ResultsThe lower detection limit of the LAMP assay was 107 viral DNA copies/μl when tested on synthesized L1 DNA sequences, which was better than the conventional PCR. Compared to PCR sequencing, the sensitivity of HPV27, HPV2, HPV1, HPV57, HPV3, HPV4, HPV7 and HPV75 genotypes detections were 100%, whereas the specificity was 34.55, 45.12, 95.83, 98.59 and 97.62% respectively, when tested on clinical samples.ConclusionsThe new cutaneous type HPV detection system is characterized by both a good sensitivity and specificity compared to conventional methods. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12985-020-01373-3 |
| 出版者 | BioMed Central |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/41028]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Sun,Yu-Zhe; Du,Yu-Guang; Gao,Xing-Hua; Qi,Rui-Qun |
| 作者单位 | 1.Dermatology Hospital of Southern Medical University 2.Chinese Academy of Sciences; Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA and State Key Laboratory of Biochemical Engineering, Institute of Process Engineering 3.Key Laboratory of Immunodermatology (China Medical University), Department of education of Liaoning Province 4.Key Laboratory of Immunodermatology 5.Key Laboratory of Immunodermatology (China Medical University), Ministry of Education 6.NHC Key Laboratory of Immunodermatology (China Medical University) 7.The First Hospital of China Medical University; Department of Dermatology |
| 推荐引用方式 GB/T 7714 | Wang,Yining,Ge,Ge,Mao,Rui,et al. Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method[J]. Virology Journal,2020,17(1). |
| APA | Wang,Yining.,Ge,Ge.,Mao,Rui.,Wang,Zhuo.,Sun,Yu-Zhe.,...&Chen,Hong-Duo.(2020).Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method.Virology Journal,17(1). |
| MLA | Wang,Yining,et al."Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method".Virology Journal 17.1(2020). |
入库方式： OAI收割
来源：过程工程研究所
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