中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A novel thrombin inhibitory peptide discovered from leech using affinity chromatography combined with ultra-high performance liquid chromatography-high resolution mass spectroscopy

文献类型:期刊论文

作者Huang, Qiuyang3; Gao, Qian3; Chai, Xiaoxin3; Ren, Wei2; Zhang, Guifeng1; Kong, Yingjun1; Zhang, Yan1; Gao, Jianping1; Lei, Xiongxin1; Ma, Li3
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2020-08-15
卷号1151页码:8
ISSN号1570-0232
关键词Thrombin Bioactive Compound Screening Affinity Chromatography Uplc-hr-ms Leech Thrombin Inhibitory Activity
DOI10.1016/j.jchromb.2020.122153
英文摘要

Thrombin (THR) inhibitors play an important role in the treatment of thrombotic diseases. This study established a THR-based bio-specific extraction coupled with affinity chromatography and ultra-high performance liquid chromatography-high resolution mass spectroscopy (UPLC-HR-MS) analysis method to screen and identify THR ligands in Leech. After evaluating the reliability of the screening method using positive control drug (hirudin), it was successfully used to screen the potential active constituents in leech. And a comprehensive analysis of the peptides in leech elution was performed by UPLC-HR-MS, a total of 34 peptides were identified. At the same time, anti-THR activity was explored and inferred by searching databases and published literature. As a result, six peptides were discovered to be potential active compounds in leech. Further, the six peptides were synthesized and in vitro enzymatic activity assay was performed. Finally, SYELPDGQVITIGNER was screened as an anti-THR peptide with an IC50, value of 255.75 mu M and it was discovered for the first time from Whitmania pigra Whitman and Hirudo nipponica Whitman. The molecular docking study showed that THR inhibitory activity of the polypeptide was mainly attributed to the hydrogen bond interactions, van der Waals forces and electrostatic interactions interaction between polypeptide and THR. These results suggest that the polypeptide is a potential natural THR inhibitor that can be used as anticoagulant.

WOS关键词Hirudin-like Factors ; Molecular Docking ; Medicinal Leech ; Ultrafiltration ; Hydrolysate ; Management ; Blood ; Drug
资助项目National Natural Science Foundation of China[81673596]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者ELSEVIER
WOS记录号WOS:000540214100001
资助机构National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/41103]  
专题中国科学院过程工程研究所
通讯作者Ma, Li
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Luzhou 646000, Peoples R China
3.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China
推荐引用方式
GB/T 7714
Huang, Qiuyang,Gao, Qian,Chai, Xiaoxin,et al. A novel thrombin inhibitory peptide discovered from leech using affinity chromatography combined with ultra-high performance liquid chromatography-high resolution mass spectroscopy[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2020,1151:8.
APA Huang, Qiuyang.,Gao, Qian.,Chai, Xiaoxin.,Ren, Wei.,Zhang, Guifeng.,...&Ma, Li.(2020).A novel thrombin inhibitory peptide discovered from leech using affinity chromatography combined with ultra-high performance liquid chromatography-high resolution mass spectroscopy.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1151,8.
MLA Huang, Qiuyang,et al."A novel thrombin inhibitory peptide discovered from leech using affinity chromatography combined with ultra-high performance liquid chromatography-high resolution mass spectroscopy".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1151(2020):8.

入库方式: OAI收割

来源:过程工程研究所

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