The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria
文献类型:期刊论文
作者 | Tracey, John C.5; Coronado, Maricela5; Giessen, Tobias W.3,4; Lau, Maggie C. Y.1,5; Silver, Pamela A.2; Ward, Bess B.5 |
刊名 | SCIENTIFIC REPORTS |
出版日期 | 2019-12-27 |
卷号 | 9页码:11 |
ISSN号 | 2045-2322 |
DOI | 10.1038/s41598-019-56533-5 |
英文摘要 | Many prokaryotes encode protein-based encapsulin nanocompartments, including anaerobic ammonium oxidizing (anammox) bacteria. This study expands the list of known anammox encapsulin systems from freshwater species to include the marine genus Scalindua. Two novel systems, identified in "Candidatus Scalindua rubra" and "Candidatus Scalindua sp. SCAELEC01 167" possess different architectures than previously studied freshwater anammox encapsulins. Characterization of the S. rubra encapsulin confirms that it can self-assemble to form compartments when heterologously expressed in Escherichia coli. BLASTp and HMMER searches of additional genomes and metagenomes spanning a range of environments returned 26 additional novel encapsulins, including a freshwater anammox encapsulin identified in "Candidatus Brocadia caroliniensis". Phylogenetic analysis comparing these 28 new encapsulin sequences and cargo to that of their closest known relatives shows that encapsulins cluster by cargo protein type and therefore likely evolved together. Lastly, prokaryotic encapsulins may be more common and diverse than previously thought. Through searching a small sample size of all public metagenomes and genomes, many new encapsulin systems were unearthed by this study. This suggests that many additional encapsulins likely remain to be discovered. |
WOS关键词 | HYDROXYLAMINE OXIDOREDUCTASE ; STRUCTURAL BASIS ; EVOLUTION ; NITROSOMONAS ; KEY ; LOCALIZATION ; ENRICHMENT ; SEARCH ; ENZYME ; TOOLS |
资助项目 | Gordon and Betty Moore Foundation[5506] |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000509340400001 |
资助机构 | Gordon and Betty Moore Foundation |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/7383] |
专题 | 深海科学研究部_地外海洋系统研究室 |
通讯作者 | Tracey, John C. |
作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China 2.Wyss Inst Biol & Inspired Engn, 3 Blackfan Circle, Boston, MA 02115 USA 3.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA 4.Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA 5.Princeton Univ, Dept Geosci, Guyot Hall, Princeton, NJ 08544 USA |
推荐引用方式 GB/T 7714 | Tracey, John C.,Coronado, Maricela,Giessen, Tobias W.,et al. The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria[J]. SCIENTIFIC REPORTS,2019,9:11. |
APA | Tracey, John C.,Coronado, Maricela,Giessen, Tobias W.,Lau, Maggie C. Y.,Silver, Pamela A.,&Ward, Bess B..(2019).The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria.SCIENTIFIC REPORTS,9,11. |
MLA | Tracey, John C.,et al."The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria".SCIENTIFIC REPORTS 9(2019):11. |
入库方式: OAI收割
来源:深海科学与工程研究所
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