Genome-centric resolution of novel microbial lineages in an excavated Centrosaurus dinosaur fossil bone from the Late Cretaceous of North America
文献类型：期刊论文
| 作者 | Liang, Renxing3; Lau, Maggie C. Y.2,3 ; Saitta, Evan T.1; Garvin, Zachary K.3; Onstott, Tullis C.3
|
| 刊名 | ENVIRONMENTAL MICROBIOME
 |
| 出版日期 | 2020-03-19 |
| 卷号 | 15期号:1页码:18 |
| 关键词 | Centrosaurus fossil bone Diagenesis Rare Actinobacteria Halotolerant bacteria Late Cretaceous Uncultured microbial lineages |
| DOI | 10.1186/s40793-020-00355-w |
| 英文摘要 | Background Exceptional preservation of endogenous organics such as collagens and blood vessels has been frequently reported in Mesozoic dinosaur fossils. The persistence of these soft tissues in Mesozoic fossil bones has been challenged because of the susceptibility of proteins to degradation and because bone porosity allows microorganisms to colonize the inner microenvironments through geological time. Although protein lability has been studied extensively, the genomic diversity of microbiomes in dinosaur fossil bones and their potential roles in bone taphonomy remain underexplored. Genome-resolved metagenomics was performed, therefore, on the microbiomes recovered from a Late Cretaceous Centrosaurus bone and its encompassing mudstone in order to provide insight into the genomic potential for microbial alteration of fossil bone. Results Co-assembly and binning of metagenomic reads resulted in a total of 46 high-quality metagenome-assembled genomes (MAGs) affiliated to six bacterial phyla (Actinobacteria, Proteobacteria, Nitrospira, Acidobacteria, Gemmatimonadetes and Chloroflexi) and 1 archaeal phylum (Thaumarchaeota). The majority of the MAGs represented uncultivated, novel microbial lineages from class to species levels based on phylogenetics, phylogenomics and average amino acid identity. Several MAGs from the classes Nitriliruptoria, Deltaproteobacteria and Betaproteobacteria were highly enriched in the bone relative to the adjacent mudstone. Annotation of the MAGs revealed that the distinct putative metabolic functions of different taxonomic groups were linked to carbon, nitrogen, sulfur and iron metabolism. Metaproteomics revealed gene expression from many of the MAGs, but no endogenous collagen peptides were identified in the bone that could have been derived from the dinosaur. Estimated in situ replication rates among the bacterial MAGs suggested that most of the microbial populations in the bone might have been actively growing but at a slow rate. Conclusions Our results indicate that excavated dinosaur bones are habitats for microorganisms including novel microbial lineages. The distinctive microhabitats and geochemistry of fossil bone interiors compared to that of the external sediment enrich a microbial biomass comprised of various novel taxa that harbor multiple gene sets related to interconnected biogeochemical processes. Therefore, the presence of these microbiomes in Mesozoic dinosaur fossils urges extra caution to be taken in the science of paleontology when hunting for endogenous biomolecules preserved from deep time. |
| WOS关键词 | MILK RIVER AQUIFER ; SP-NOV ; PROTEIN SEQUENCES ; DNA PRESERVATION ; IDENTIFICATION ; ANNOTATION ; NITROSPIRA ; EVOLUTION ; BACTERIA ; ALBERTA |
| 资助项目 | Scott Vertebrate Paleontology Fund from the Department of Geosciences, Princeton University |
| WOS研究方向 | Genetics & Heredity ; Microbiology |
| 语种 | 英语 |
| 出版者 | BMC |
| WOS记录号 | WOS:000521494700001 |
| 资助机构 | Scott Vertebrate Paleontology Fund from the Department of Geosciences, Princeton University |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/7524]  |
| 专题 | 深海科学研究部_地外海洋系统研究室 |
| 通讯作者 | Liang, Renxing |
| 作者单位 | 1.Field Museum Nat Hist, Integrat Res Ctr, Sect Earth Sci, Chicago, IL 60605 USA 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Peoples R China 3.Princeton Univ, Dept Geosci, B88,Guyot Hall, Princeton, NJ 08544 USA |
| 推荐引用方式 GB/T 7714 | Liang, Renxing,Lau, Maggie C. Y.,Saitta, Evan T.,et al. Genome-centric resolution of novel microbial lineages in an excavated Centrosaurus dinosaur fossil bone from the Late Cretaceous of North America[J]. ENVIRONMENTAL MICROBIOME,2020,15(1):18. |
| APA | Liang, Renxing,Lau, Maggie C. Y.,Saitta, Evan T.,Garvin, Zachary K.,&Onstott, Tullis C..(2020).Genome-centric resolution of novel microbial lineages in an excavated Centrosaurus dinosaur fossil bone from the Late Cretaceous of North America.ENVIRONMENTAL MICROBIOME,15(1),18. |
| MLA | Liang, Renxing,et al."Genome-centric resolution of novel microbial lineages in an excavated Centrosaurus dinosaur fossil bone from the Late Cretaceous of North America".ENVIRONMENTAL MICROBIOME 15.1(2020):18. |
入库方式： OAI收割
来源：深海科学与工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。