Insights into high-pressure acclimation: comparative transcriptome analysis of sea cucumber Apostichopus japonicus at different hydrostatic pressure exposures
文献类型：期刊论文
| 作者 | Liang, Linying1,2 ; Chen, Jiawei1,2; Li, Yanan1,2; Zhang, Haibin2
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| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2020-01-21 |
| 卷号 | 21期号:1页码:11 |
| 关键词 | Hydrostatic pressure Acclimation Transcriptome Differentially expressed gene Sea cucumber |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-020-6480-9 |
| 英文摘要 | Background Global climate change is predicted to force the bathymetric migrations of shallow-water marine invertebrates. Hydrostatic pressure is proposed to be one of the major environmental factors limiting the vertical distribution of extant marine invertebrates. However, the high-pressure acclimation mechanisms are not yet fully understood. Results In this study, the shallow-water sea cucumber Apostichopus japonicus was incubated at 15 and 25 MPa at 15 degrees C for 24 h, and subjected to comparative transcriptome analysis. Nine samples were sequenced and assembled into 553,507 unigenes with a N50 length of 1204 bp. Three groups of differentially expressed genes (DEGs) were identified according to their gene expression patterns, including 38 linearly related DEGs whose expression patterns were linearly correlated with hydrostatic pressure, 244 pressure-sensitive DEGs which were up-regulated at both 15 and 25 MPa, and 257 high-pressure-induced DEGs which were up-regulated at 25 MPa but not up-regulated at 15 MPa. Conclusions Our results indicated that the genes and biological processes involving high-pressure acclimation are similar to those related to deep-sea adaptation. In addition to representative biological processes involving deep-sea adaptation (such as antioxidation, immune response, genetic information processing, and DNA repair), two biological processes, namely, ubiquitination and endocytosis, which can collaborate with each other and regulate the elimination of misfolded proteins, also responded to high-pressure exposure in our study. The up-regulation of these two processes suggested that high hydrostatic pressure would lead to the increase of misfolded protein synthesis, and this may result in the death of shallow-water sea cucumber under high-pressure exposure. |
| WOS关键词 | TEMPERATURE ; SHALLOW ; ADAPTATION ; GENE ; EXTINCTIONS ; TOLERANCE ; PROTEINS ; CLIMATE ; HSP70 ; COLD |
| 资助项目 | Strategic Priority Research Program of CAS[XDA22040502] ; National Key Research and Development Program of China[2016YFC0304905] ; National Key Research and Development Program of China[2018YFC0309804] ; Major scientific and technological projects of Hainan Province[ZDKJ2016012] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000513652600003 |
| 出版者 | BMC |
| 资助机构 | Strategic Priority Research Program of CAS ; National Key Research and Development Program of China ; Major scientific and technological projects of Hainan Province |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/7489]  |
| 专题 | 研究生部 深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组 |
| 通讯作者 | Zhang, Haibin |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Linying,Chen, Jiawei,Li, Yanan,et al. Insights into high-pressure acclimation: comparative transcriptome analysis of sea cucumber Apostichopus japonicus at different hydrostatic pressure exposures[J]. BMC GENOMICS,2020,21(1):11. |
| APA | Liang, Linying,Chen, Jiawei,Li, Yanan,&Zhang, Haibin.(2020).Insights into high-pressure acclimation: comparative transcriptome analysis of sea cucumber Apostichopus japonicus at different hydrostatic pressure exposures.BMC GENOMICS,21(1),11. |
| MLA | Liang, Linying,et al."Insights into high-pressure acclimation: comparative transcriptome analysis of sea cucumber Apostichopus japonicus at different hydrostatic pressure exposures".BMC GENOMICS 21.1(2020):11. |
入库方式： OAI收割
来源：深海科学与工程研究所
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