Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases
文献类型：期刊论文
| 作者 | Ali, Nasir2,3,4; Giwa, Abdulmoseen Segun1; Abdalla, Mohnad2; Liu, Xiang3 |
| 刊名 | Cellulose
 |
| 出版日期 | 2020 |
| 卷号 | 27期号:2页码:769-779 |
| ISSN号 | 09690239 |
| DOI | 10.1007/s10570-019-02829-8 |
| 英文摘要 | Abstract: Bamboo (Phyllostachys acuta) is considered one of the useful feedstocks of crop residues due to speedy growth, fastest propagation, and convenient harvesting. The influence of alkaline hydrogen peroxide (AHP) pretreatment of bamboo culm, its structural changes, and enzymatic hydrolysis were determined. Scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction were used for the analysis of solid fraction after alkaline hydrogen peroxide treatment of bamboo culm. Recombinant enzymes were expressed in Pichia pastoris from newly identified Aspergillus niger BE-2. The obtained results revealed more hemicelluloses hydrolysis and improved cellulose accumulation in degraded part. The cellulose component was increased by 36.87%, hemicellulose decreased by 50.66%, and lignin by 37.94% in comparison with the chemical components in the raw material after AHP pretreatment. There is 111% increased yield reported for recombinant enzymes expressed in Pichia pastoris after 60 h of degradation as compared to untreated substrates biomass. A total of about 370 mg reducing sugars per gram dehydrated bamboo residues were obtained after AHP treatment. The results revealed that major structural changes take place in the physiology of the substrates after AHP treatment, including elimination of lignin and hemicellulose, and enhance the porous area for easy attack of recombinant cellulases. This investigation contributes in biomass conversion in a friendly environment to fulfil the energy requirement in the future challenges. Graphic abstract: [Figure not available: see fulltext.] © 2019, Springer Nature B.V. |
| 电子版国际标准刊号 | 1572882X |
| 语种 | 英语 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/9864]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 作者单位 | 1.Green Intelligence Environmental School, Yangtze Normal University, Chongqing; 408003, China 2.Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao; Shandong Province; 266101, China; 3.State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing; 100084, China; 4.School of Life Science, Xiamen University, Xiamen; 361005, China; |
| 推荐引用方式 GB/T 7714 | Ali, Nasir,Giwa, Abdulmoseen Segun,Abdalla, Mohnad,et al. Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases[J]. Cellulose,2020,27(2):769-779. |
| APA | Ali, Nasir,Giwa, Abdulmoseen Segun,Abdalla, Mohnad,&Liu, Xiang.(2020).Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases.Cellulose,27(2),769-779. |
| MLA | Ali, Nasir,et al."Alkaline hydrogen peroxide pretreatment of bamboo culm for improved enzymatic release of reducing sugars using recombinant cellulases".Cellulose 27.2(2020):769-779. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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