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Cellular fate of deformable needle-shaped PLGA-PEG fibers

文献类型：期刊论文

作者Zhang BK6,8; Zhu ML(朱鸣柳)2,7; Li Z3,6; Lung PS8; Chrzanowski W5; Kwok CT4; Lu J1,7; Li Q8
刊名ACTA BIOMATERIALIA
出版日期2020-08-01
卷号112页码:182-189
关键词Deformability Apparent Young's modulus Actin revolution
ISSN号1742-7061
DOI10.1016/j.actbio.2020.05.029
英文摘要Deformability of micro/nanometer sized particles plays an important role in particle-cell interactions and thus becomes a key parameter in carrier design in biomedicine application such as drug delivery and vaccinology. Yet the influence of material's deformability on the cellular fate of the particles as well as physiology response of live cells are to be understood. Here we show the cellular fate of needle shaped (high aspect ratio similar to 25) PLGA-PEG copolymer fibers depending on their deformability. We found that all the fibers entered murine macrophage cells (RAW 264.7) via phagocytosis. While the fibers of high apparent Young's modulus (average value = 872 kPa) maintained their original shape upon phagocytosis, their counterparts of low apparent Young's modulus (average value = 56 kPa) curled in cells. The observed deformation of fibers of low apparent Young's modulus in cells coincided with abnormal intracellular actin translocation and absence of lysosome/phagosome fusion in macrophages, suggesting the important role of material mechanical properties and mechano-related cellular pathway in affecting cell physiology. Statement of Significance Particles are increasingly important in the field of biomedicine, especially when they are serving as drug carriers. Physical cues, such as mechanical properties, were shown to provide insight into their stability and influence on physiology inside the cell. In the current study, we managed to fabricate 5 types of needle shaped PLGA-PEG fibers with controlled Young's modulus. We found that hard fibers maintained their original shape upon phagocytosis, while soft fibers were curled by actin compressive force inside the cell, causing abnormal actin translocation and impediment of lysosome/phagosome fusion, suggesting the important role of material mechanical properties and mechano-related cellular pathway in affecting cell physiology. (c) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
分类号一类
WOS关键词NANOPARTICLE SIZE ; SURFACE-CHARGE ; DRUG-DELIVERY ; ACTIN ; FORCES ; POLYMERIZATION ; REQUIREMENTS ; PHAGOCYTOSIS ; CIRCULATION ; PARTICLES
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000549500900015
资助机构Hong Kong Collaborative Research Fund (CRF) Scheme[C4026-17W] ; Theme-based Research Scheme[T13-402/17-N]
其他责任者Lu, Jian ; Li, Quan
源URL[http://dspace.imech.ac.cn/handle/311007/84738]  
专题力学研究所_非线性力学国家重点实验室
作者单位1.City Univ Hong Kong, Ctr Adv Struct Mat, Greater Bay Joint Div, Shenyang Natl Lab Mat Sci,Shenzhen Res Inst, Shenzhen 518057, Peoples R China
2.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, 15 West Rd,North 4th Ring, Beijing 100190, Peoples R China;
3.Nanjing Univ Aeronaut & Astronaut, State Key Lab Mech & Control Mech Struct, Nanjing 210016, Jiangsu, Peoples R China;
4.Univ Macau, Dept Electromech Engn, Zhuhai, Australia;
5.Univ Sydney, Fac Pharm, Sydney, NSW, Australia;
6.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Key Lab Nanobiomech, Beijing, Peoples R China;
7.City Univ Hong Kong, Dept Mech Engn, Kowloon, Hong Kong, Peoples R China;
8.Chinese Univ Hong Kong, Dept Phys, Hong Kong, Peoples R China;
推荐引用方式
GB/T 7714		 Zhang BK,Zhu ML,Li Z,et al. Cellular fate of deformable needle-shaped PLGA-PEG fibers[J]. ACTA BIOMATERIALIA,2020,112:182-189.
APA Zhang BK.,朱鸣柳.,Li Z.,Lung PS.,Chrzanowski W.,...&Li Q.(2020).Cellular fate of deformable needle-shaped PLGA-PEG fibers.ACTA BIOMATERIALIA,112,182-189.
MLA Zhang BK,et al."Cellular fate of deformable needle-shaped PLGA-PEG fibers".ACTA BIOMATERIALIA 112(2020):182-189.

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来源：力学研究所

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