Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application
文献类型：期刊论文
| 作者 | Huang CH4; Wang BX3; Chen YY(陈艳艳)2 ; Jin G(靳刚)1
|
| 刊名 | INTEGRATED FERROELECTRICS
 |
| 出版日期 | 2020-07-23 |
| 卷号 | 209期号:1页码:19-29 |
| 关键词 | Imaging ellipsometry carcinoembryonic antigen immunoassay |
| ISSN号 | 1058-4587 |
| DOI | 10.1080/10584587.2020.1728800 |
| 英文摘要 | This study represents a label-free immunoassay for carcinoembryonic antigen (CEA) by microfluidic biosensor based on imaging ellipsometry (BIE) and its application is further validated by clinic sera. The sandwich format of analysis configuration was adopted, in which Rat-mAb was served as capture antibody, rabbit anti-CEA polyclonal antibody (rabbit-pAb) as detection antibody and goat anti-rabbit IgG (goat-IgG) as an enhancer added to increase detection signal. The methodology includes rat-mAb concentration screening, rabbit-pAb and goat-IgG concentration optimization, creation of dose-response curve and determination of cutoff value. Results show the linear range is from 4.0-64.0 ng/ml with a lower limit of 1.0 ng/ml (S/N = 3). Application of the BIE to clinic samples demonstrated that the relation coefficient is 0.94 and substantial agreement with the results of ECLIA's (Kappa = 0.733,U = 4.35 > U-0.01). The proposed method exhibits the potential for application. |
| 分类号 | Q4 |
| WOS关键词 | FLOW-INJECTION IMMUNOASSAY ; OFF-NULL ELLIPSOMETRY ; IMMUNOSENSOR ; VISUALIZATION ; VALIDATION ; LAYERS ; ASSAY |
| WOS研究方向 | Engineering ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000547668500003 |
| 资助机构 | Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism[LIFM201712] ; NSF of Chongqing[cstc2016shmszx80080] ; NSF of Chongqing[cstc2019jscx-msxm1542] ; Chongqing Education Board[KJ1605701] |
| 其他责任者 | Huang, Chenghong |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/84744]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Beijing, Peoples R China; 3.Chongqing Light Ind Polytech Coll, Jiulongpo, Peoples R China; 4.Chongqing Univ Sci & Technol, Chongqing Key Lab Ind Fermentat Microorganism, Shapingba 401331, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Huang CH,Wang BX,Chen YY,et al. Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application[J]. INTEGRATED FERROELECTRICS,2020,209(1):19-29. |
| APA | Huang CH,Wang BX,陈艳艳,&靳刚.(2020).Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application.INTEGRATED FERROELECTRICS,209(1),19-29. |
| MLA | Huang CH,et al."Label-Free Immunoassay of Carcinoembryonic Antigen by Microfluidic Channel Biosensor Based on Imaging Ellipsometry and Its Clinic Application".INTEGRATED FERROELECTRICS 209.1(2020):19-29. |
入库方式： OAI收割
来源：力学研究所
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