Regulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography
文献类型：会议论文
| 作者 | Lv DY(吕东媛)
|
| 出版日期 | 2019-06-27 |
| 会议日期 | 2019-06-27 |
| 会议地点 | 中国江苏常州 |
| 关键词 | Regulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography |
| 页码 | 46 |
| 英文摘要 | Results and Discussion:Hepatic differentiation of hESCs on topographical PA hydrogel with two typical stiffnesses was systematically tested using a four-stage induction protocol,we proposed a working model to illustrate how the two mechanical factors cooperatively manipulate the hepatic differentiation of hESCs.Substrate stiffness and topography are critical in regulating cell functions,coupled impacts of topography and stiffness were found differentially at distinct differentiation stages.Substrate stiffness is dominant in sternness maintenance and defined endoderm differentiation.Substrate topography plays a decisive role in manipulating clone or cell morphology and spreading and favors the differentiation to hepatocyte-like cells formation and maturation.Both substrate stiffness and topography cooperatively support clone formation or cell shape maintenance,and the differentiated cells present liver-specific functions,such as albumin excretion,glycogen synthesis,and ICG engulfment.Conclusions:These results indicate that stem cell morphology and differentiation are affected by substrate stiffness and topography that mimics physiological conditions,combined with those biochemical factors.Biomechanical cues are critical for enhancing the efficiency and maturity of hepatic differentiation of ESCs. |
| 会议录 | 中国生理学会基质生物学专业委员会第四次全国基质生物学学术会议会议手册
 |
| 语种 | 英语 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/84945]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 中国科学院力学研究所 |
| 推荐引用方式 GB/T 7714 | Lv DY. Regulation of morphology and differentiation of embryonic stem cells by substrate stiffness and topography[C]. 见:. 中国江苏常州. 2019-06-27. |
入库方式： OAI收割
来源：力学研究所
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