Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression
文献类型：期刊论文
| 作者 | Zhang F(张帆)1,2,3 ; Wang JW(王家文)1,2,3; Lv DY(吕东媛)1,2,3; Zheng L(郑璐)1,2,3; Shang GB(上官冰)2,3; Gao YX(高宇欣)2,3; Wu Y(武亿)1,2,3; Long M(龙勉)1,2,3
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| 刊名 | BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
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| 出版日期 | 2020-08-18 |
| 页码 | 18 |
| 关键词 | Mechanomics hESCs iTRAQ GSEA WGCNA |
| ISSN号 | 1617-7959 |
| DOI | 10.1007/s10237-020-01378-5 |
| 通讯作者 | Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Human embryonic stem cells (hESCs) can differentiate to three germ layers within biochemical and biomechanical niches. The complicated mechanical environments in vivo could have diverse effects on the fate decision and biological functions of hESCs. To globally screen mechanosensitive molecules, three typical types of mechanical stimuli,i.e.,tensile stretch, shear flow, and mechanical compression, were applied in respective parameter sets of loading pattern, amplitude, frequency, and/or duration, and then, iTRAQ proteomics test was used for identifying and quantifying differentially expressed proteins in hESCs. Bioinformatics analysis identified 37, 41, and 23 proteins under stretch pattern, frequency, and duration, 13, 18, and 41 proteins under shear pattern, amplitude, and duration, and 4, 0, and 183 proteins under compression amplitude, frequency, and duration, respectively, where distinct parameters yielded the differentially weighted preferences under each stimulus. Ten mechanosensitive proteins were commonly shared between two of three mechanical stimuli, together with numerous proteins identified under single stimulus. More importantly, functional GSEA and WGCNA analyses elaborated the variations of the screened proteins with loading parameters. Common functions in protein synthesis and modification were identified among three stimuli, and specific functions were observed in skin development under stretch alone. In conclusion, mechanomics analysis is indispensable to map actual mechanosensitive proteins under physiologically mimicking mechanical environment, and sheds light on understanding the core hub proteins in mechanobiology. |
| 分类号 | 二类 |
| WOS关键词 | EMBRYONIC STEM-CELLS ; NUCLEAR-LOCALIZATION ; ARTICULAR-CARTILAGE ; STRESS ; DIFFERENTIATION ; EXPRESSION ; EXPANSION ; FORCES ; MORPHOGENESIS ; PROLIFERATION |
| 资助项目 | National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31470907] ; Frontier Science Key Project of Chinese Science Academy grant[QYZDJ-SSW-JSC018] |
| WOS研究方向 | Biophysics ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000560629600001 |
| 资助机构 | National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy grant |
| 其他责任者 | Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/84971]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang F,Wang JW,Lv DY,et al. Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression[J]. BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,2020:18. |
| APA | 张帆.,王家文.,吕东媛.,郑璐.,上官冰.,...&龙勉.(2020).Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression.BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,18. |
| MLA | 张帆,et al."Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression".BIOMECHANICS AND MODELING IN MECHANOBIOLOGY (2020):18. |
入库方式： OAI收割
来源：力学研究所
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