A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula
文献类型：期刊论文
| 作者 | Wang, Weidong; Dang, Jun; Shao, Yun; Jiang, Lei; Liu, Zenggen ; Mei, Lijuan; Tao, Yanduo
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2020 |
| 卷号 | 43期号:21 |
| 关键词 | flavonoid glycosides large volume injection online enrichment preparative chromatography traditional medicine |
| 英文摘要 | Flavonoid glycosides exist widely in medicine herbs and often used as nutraceuticals because of their excellent bioactivity and low toxicity. For accurate quality control and bioactivity assessment of Sphaerophysa salsula, a rapid and productive method to isolate flavonoid glycosides is needed. Therefore, this work reports the development of a novel comprehensive strategy based on an online middle-pressure chromatography and preparative high-performance liquid chromatography for rapid enrichment and separation of flavonoid glycosides from S. salsula. First, the flavonoid glycosides were enriched using an online middle-pressure chromatographic column containing stationary middle chromatogram isolated phase. During this process, the high-volume injection of the extracting solution was realized by an empty precolumn positioned before the main chromatographic tower. Then, the compounds were separated through preparative high-performance liquid chromatography with Megress C18. As a result, one new flavonol 3-O-glycoside (2) and two known flavonol 3-O-glycosides (1, 3) were targetedly isolated from S. salsula. The content of compounds 1-3 in S. salsula was 0.09, 0.11, and 0.18 wt%, respectively. Comparing to traditional enrichment and separation methods, our technique offers significantly shorter sample pretreatment time as well as high reproducibility. We believe that our separation method has a strong potential to be used for the processing of other medicinal plants. |
| 源URL | [http://210.75.249.4/handle/363003/60218]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Weidong,Dang, Jun,Shao, Yun,et al. A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula[J]. JOURNAL OF SEPARATION SCIENCE,2020,43(21). |
| APA | Wang, Weidong.,Dang, Jun.,Shao, Yun.,Jiang, Lei.,Liu, Zenggen.,...&Tao, Yanduo.(2020).A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula.JOURNAL OF SEPARATION SCIENCE,43(21). |
| MLA | Wang, Weidong,et al."A novel chromatographic separation method for rapid enrichment and isolation of novel flavonoid glycosides from Sphaerophysa salsula".JOURNAL OF SEPARATION SCIENCE 43.21(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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