Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7
文献类型：期刊论文
| 作者 | Wang, Zonghan; Yao, Xiaolin; Zhang, Yongzhi; Wang, Rong; Ji, Yanwei; Sun, Jing ; Zhang, Daohong; Wang, Jianlong
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| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2020 |
| 卷号 | 329 |
| 关键词 | Label-free Multi-readout Nanozyme Lateral flow immunoassay Mannose modified Prussian blue Escherichia coli O157:H7 |
| 英文摘要 | To overcome the drawbacks of antibody labeling dependence and single-readout system in the conventional lateral flow immunoassays (LFIAs) as well as the non-targeted combination of new capture agents reported recently for pathogen detection, in this work, a multi-readout and label-free LFIA was proposed for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) based on a nanozyme-bacteria-antibody sandwich pattern. A type of functional nanozyme-mannose modified Prussian blue (man-PB), was introduced as the recognition agent as well as signal indicator. Apart from original signal intensity on the T-line, the peroxidase-like catalytic activity-driven generation of colorimetric signal could be used as another format of quantitation. Importantly, such LFIA could exhibit excellent performance for target pathogens detection separately with a quantitative range of 10(2)-10(8) cfu.mL(-1) and a low detection limit of 10(2) cfu.mL(-1) based on different readout formats, indicating the application potential of the proposed LFIA in real samples. |
| 源URL | [http://210.75.249.4/handle/363003/60221]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Zonghan,Yao, Xiaolin,Zhang, Yongzhi,et al. Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7[J]. FOOD CHEMISTRY,2020,329. |
| APA | Wang, Zonghan.,Yao, Xiaolin.,Zhang, Yongzhi.,Wang, Rong.,Ji, Yanwei.,...&Wang, Jianlong.(2020).Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7.FOOD CHEMISTRY,329. |
| MLA | Wang, Zonghan,et al."Functional nanozyme mediated multi-readout and label-free lateral flow immunoassay for rapid detection of Escherichia coli O157:H7".FOOD CHEMISTRY 329(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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