Separation of five flavonoids with similar polarity fromCaragana korshinskiiKom. by preparative high speed counter-current chromatography with recycling and heart cut mode
文献类型：期刊论文
| 作者 | Ma, Yumei; Yang, Xue; Chen, Jilin; Zhao, Jingyang; Yang, Li; Yan, Shuping ; Li, Hongmei; Shen, Cheng; Wei, Yangfei; Wang, Shuo
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2020 |
| 关键词 | Caragana korshinskiiKom flavonoid glycosides high-speed counter-current chromatography recycling similar polarity |
| 英文摘要 | Five flavonoids with similar polarity were obtained fromCaragana korshinskiiKom. by preparative high speed counter-current chromatography. Due to their similar polarity, it is difficult to select a suitable solvent system for one-step separation. Three fractions including one pure compound were only obtained with a relatively suitable solvent system of ethyl acetate:n-butanol:water (0.5% glacial acetic acid) (4:6:10, v:v:v). In order to improve the separation efficiency, recycling and heart cut modes were introduced. As a result, two flavonoids with separation factor value at 1.13 and the other two with separation factor value at 1.20 were successfully separated. Finally, five flavonoids with purity higher than 98% were obtained. Fortunately, a new compound named myricetin 3 '-methyl ether 3-O-glucoside-7-O-rhamnoside was obtained. The results indicated that the recycling combined with heart-cut mode could be effective for the preparation of natural compounds with similar polarity. |
| 源URL | [http://210.75.249.4/handle/363003/60277]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Ma, Yumei,Yang, Xue,Chen, Jilin,et al. Separation of five flavonoids with similar polarity fromCaragana korshinskiiKom. by preparative high speed counter-current chromatography with recycling and heart cut mode[J]. JOURNAL OF SEPARATION SCIENCE,2020. |
| APA | Ma, Yumei.,Yang, Xue.,Chen, Jilin.,Zhao, Jingyang.,Yang, Li.,...&Li, Yulin.(2020).Separation of five flavonoids with similar polarity fromCaragana korshinskiiKom. by preparative high speed counter-current chromatography with recycling and heart cut mode.JOURNAL OF SEPARATION SCIENCE. |
| MLA | Ma, Yumei,et al."Separation of five flavonoids with similar polarity fromCaragana korshinskiiKom. by preparative high speed counter-current chromatography with recycling and heart cut mode".JOURNAL OF SEPARATION SCIENCE (2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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