Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening
文献类型：期刊论文
| 作者 | Fang, Yi; Zhao, Chengzhen; Xiang, Hai; Jia, GongXue; Zhong, Rongzhen |
| 刊名 | REPRODUCTION IN DOMESTIC ANIMALS
 |
| 出版日期 | 2020 |
| 卷号 | 55期号:9 |
| 关键词 | cryopreservation melatonin mitochondrial permeability transition pore ram sperm |
| 英文摘要 | Cryopreservation damages permeability of sperm mitochondrial membranes, with formation of a mitochondrial permeability transition pore (mPTP). Mitochondria are both a primary synthesis site and principle target for melatonin, which can directly inhibit mPTP formation. The objective was to determine effects of melatonin on mPTP opening of frozen-thawed ram sperm and elucidate underlying pathways by antagonist and agonists of melatonin receptors (MTs), and antagonists of PI3K and GSK 3 beta treatments; furthermore, plasma membrane integrity, mitochondrial membrane potential (Delta psi m), mitochondrial cytochrome c (Cyt c) release and fertilization were analysed to assess the effect of mPTP status mediated by melatonin on quality of frozen-thawed sperm. Fresh ram semen was diluted in glucose-egg yolk buffer with 0 or 10(-7) M melatonin (frozen and frozen + melatonin groups, respectively) and slow-frozen. In frozen-thawed sperm, melatonin added at initiation of 4 degrees C equilibration was most effective for inhibiting mPTP opening, decreasing peptidyl-prolyl-cis/trans isomerase activity of cyclophilin D and increasing plasma membrane integrity, Delta psi m, mitochondrial Cyt c concentration and fertilizing ability (p .05). In a mechanistic study, the melatonin receptor (MT)1 antagonist eliminated inhibition of melatonin on mPTP opening, whereas MT1 agonist had opposite effects (p < .05). Neither MT2 antagonist nor agonist had significant effect, but PI3K and/or GSK 3 beta antagonist decreased inhibition of MT1 agonist on mPTP opening (p .05). In conclusion, melatonin improved sperm cryopreservation, perhaps by acting on MT1 via the PI3K-Akt-GSK 3 beta pathway to inhibit mPTP opening. |
| 源URL | [http://210.75.249.4/handle/363003/60280]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Fang, Yi,Zhao, Chengzhen,Xiang, Hai,et al. Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening[J]. REPRODUCTION IN DOMESTIC ANIMALS,2020,55(9). |
| APA | Fang, Yi,Zhao, Chengzhen,Xiang, Hai,Jia, GongXue,&Zhong, Rongzhen.(2020).Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening.REPRODUCTION IN DOMESTIC ANIMALS,55(9). |
| MLA | Fang, Yi,et al."Melatonin improves cryopreservation of ram sperm by inhibiting mitochondrial permeability transition pore opening".REPRODUCTION IN DOMESTIC ANIMALS 55.9(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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