9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine
文献类型：期刊论文
| 作者 | Sun, Luping; Zhu, Shuyun; Zheng, Zhenjia; Sun, Jing ; Zhao, Xian-En; Liu, Huwei
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2020 |
| 卷号 | 1623 |
| 关键词 | Derivatization Hydroxyl polycyclic aromatic hydrocarbons Exposome High throughput Multiplexed tags chemical isotope labeling Magnetic dispersive solid phase extraction |
| 英文摘要 | Hydroxyl-polycyclic aromatic hydrocarbons (OH-PAHs) are biomarkers for assessing the exposure levels of polycyclic aromatic hydrocarbons (PAHs). A series of stable isotope mass tags (SIMT-332/338/346/349/351/354/360/363/374/377) were firstly designed and synthesized to perform multiplexed stable isotope labeling derivatization (MSILD) of OH-PAHs in human plasma and urine. Their derivatives were enriched and purified by magnetic dispersive solid phase extraction (MDSPE) using prepared Fe3O4/GO and then determined by ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) in multiple reaction monitoring mode. 9-Plexed MSILD reagents were prepared using pipemidic acid as core structure with different isotope mass tags, in which carbonyl chloride group was used to label OH-PAHs. The SIMT-346 labeled OH-PAHs standards were used as internal standards, which can greatly increase the quantitative accuracy. 9-Plex labeled nine different real samples can be quantified by UHPLC-MS/MS in a single run. Under optimized MSILD-MDSPE conditions, good linearities of seven OH-PAHs were obtained with satisfactory coefficient of determination R-2 > 0.991. Limits of detection (LODs) of seven OH-PAHs were from 0.1 to 0.5 pg/mL, and limits of quantitation (LOQs) ranged from 0.5 to 2.0 pg/mL. The intra- and inter-day precisions ranged in 2.3-12.4% with accuracies in the range of 91.7-108.4%. Acceptable results of matrix effect (89.7-105.7%) and derivatization efficiency (> 96.4%) were obtained. In short, the developed method has been proved to be high-throughput, sensitive, accurate and easy-handling. This method was applied for the measurement of seven free OH-PAHs in human urine and plasma, and expected to provide technical support for the evaluation of PAHs exposure levels in humans. (C) 2020 Elsevier B.V. All rights reserved. |
| 源URL | [http://210.75.249.4/handle/363003/60292]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Luping,Zhu, Shuyun,Zheng, Zhenjia,et al. 9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1623. |
| APA | Sun, Luping,Zhu, Shuyun,Zheng, Zhenjia,Sun, Jing,Zhao, Xian-En,&Liu, Huwei.(2020).9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine.JOURNAL OF CHROMATOGRAPHY A,1623. |
| MLA | Sun, Luping,et al."9-Plex ultra high performance liquid chromatography tandem mass spectrometry determination of free hydroxyl polycyclic aromatic hydrocarbons in human plasma and urine".JOURNAL OF CHROMATOGRAPHY A 1623(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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