Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation
文献类型：期刊论文
| 作者 | Hu, Jingwen; Zhu, Shuyun; Chen, Shi-En; Liu, Ruisheng; Sun, Jing ; Zhao, Xian-En; Liu, Huwei
|
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2020 |
| 卷号 | 187期号:7 |
| 关键词 | Globotriaosylsphingosine Fabry disease biomarker Multiplexed labeling Dummy molecular imprinting technology Magnetic material Triple quadrupole mass spectrometry |
| 英文摘要 | A new series of 9-plex chemical isotope-labeling reagents, levofloxacin-based mass tags (LMTs) named as LMT359, 360, 361, 362, 363, 373, 375, 376, and 378, was firstly designed and synthesized for the high-throughput labeling of globotriaosylsphingosine (lyso-Gb3), a disease biomarker of Fabry disease. Creatively based on derivatization strategy-dummy template technique, dummy magnetic molecularly imprinted polymers (DMMIPs) were designed and prepared using LMT387-labeled lyso-Gb3 as a dummy template. The novel DMMIP material was used as sorbents for magnetic dispersive solid-phase extraction of 9-plexed LMT derivatives of lyso-Gb3 from equally mixed derivatization solutions. The enriched 8-plexed lyso-Gb3 derivatives from 8 real samples were quantified by ultra-high-performance liquid chromatography tandem mass spectrometry in a single run using simultaneously extracted LMT359-labeled standard lyso-Gb3 as internal standards. DMMIPs were characterized by using the transmission electron microscope (TEM), Fourier transform infrared, X-ray photoelectron spectroscopy, and some other characterization techniques. TEM micrograph showed that the prepared DMMIPs had an apparent imprinting layer. Triple-recognition abilities of DMMIPs towards LMT-lyso-Gb3 mainly rely on the hydrogen bonding, electrostatic attraction, hydrophobic interaction, and boronate affinity. The imprinting factor of DMMIPs towards LMT-lyso-Gb3 was 5.1. This method shows the advantages of high selectivity (triple recognition), high sensitivity, high accuracy (recovery 93.5-108.8%), and high throughput (8 samples in a single run). The proposed method was successfully applied to the determination of lyso-Gb3 in plasma samples with spiked recoveries in the range of 95.0-102.4%. This indicates that the method is promising in bioanalysis and medical testing of lyso-Gb3 in the future.Synthesis of multiplexed derivatization reagents and its correlative molecularly imprinted polymers for magnetic extraction of globotriaosylsphingosine |
| 源URL | [http://210.75.249.4/handle/363003/60315]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Hu, Jingwen,Zhu, Shuyun,Chen, Shi-En,et al. Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation[J]. MICROCHIMICA ACTA,2020,187(7). |
| APA | Hu, Jingwen.,Zhu, Shuyun.,Chen, Shi-En.,Liu, Ruisheng.,Sun, Jing.,...&Liu, Huwei.(2020).Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation.MICROCHIMICA ACTA,187(7). |
| MLA | Hu, Jingwen,et al."Multiplexed derivatization strategy-based dummy molecularly imprinted polymers as sorbents for magnetic dispersive solid phase extraction of globotriaosylsphingosine prior to UHPLC-MS/MS quantitation".MICROCHIMICA ACTA 187.7(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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