Preparative separation of isoquinoline alkaloids from Corydalis impatiens using middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography
文献类型：期刊论文
| 作者 | Wang, Qi; Chen, Wenjie; Wang, Qilan ; Tao, Yanduo; Yu, Ruitao; Pan, Guoqing; Dang, Jun
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2020 |
| 卷号 | 43期号:13 |
| 关键词 | isoquinoline alkaloids preparative separation positive charge traditional Tibetan medicine |
| 英文摘要 | Positively charged reversed-phase liquid chromatography was employed for the efficient preparative separation of isoquinoline alkaloids from Corydalis impatiens. Ten commercially available columns were compared for isoquinoline alkaloids analysis. While tailing, overloading, lower resolution, and buffer salts limited the application in purification of isoquinoline compounds of many of these columns, one positively charged reversed-phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading isoquinoline compounds on a larger, preparative scale. The general separation process is as follows. First, isoquinoline alkaloids are enriched with Corydalis impatiens extract via a middle chromatogram isolated gel column. After column selection, separation is performed on an XCharge C18 analytical column, from which two evident chromatographic peaks are readily obtained. Finally, two isoquinoline alkaloids (protopine and corydamine) are selectively purified on the XCharge C18 preparative column. These results demonstrate that a middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography is effective for the preparative separation of isoquinoline alkaloids from Corydalis impatiens. |
| 源URL | [http://210.75.249.4/handle/363003/60334]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Qi,Chen, Wenjie,Wang, Qilan,et al. Preparative separation of isoquinoline alkaloids from Corydalis impatiens using middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2020,43(13). |
| APA | Wang, Qi.,Chen, Wenjie.,Wang, Qilan.,Tao, Yanduo.,Yu, Ruitao.,...&Dang, Jun.(2020).Preparative separation of isoquinoline alkaloids from Corydalis impatiens using middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography.JOURNAL OF SEPARATION SCIENCE,43(13). |
| MLA | Wang, Qi,et al."Preparative separation of isoquinoline alkaloids from Corydalis impatiens using middle chromatogram isolated gel column coupled with positively charged reversed-phase liquid chromatography".JOURNAL OF SEPARATION SCIENCE 43.13(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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