Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects
文献类型：期刊论文
| 作者 | Zhang, Xinxin; Wang, Xingbin; Khurm, Muhammad; Zhan, Guanqun; Zhang, Hui; Ito, Yoichiro; Guo, Zengjun |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2020 |
| 卷号 | 19期号:3 |
| 关键词 | diosgenin cerebral ischemic reperfusion anti-inflammatory STAT2 HIKESHI |
| 英文摘要 | Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in the pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment remain elusive. In the present study, the proteomics analysis of the brain tissues from I/R rats after DIO treatment was performed using an integrated TMT-based quantitative proteomic approach coupled with the liquid chromatography with tandem mass spectrometry technology. A total of 5043 proteins (ProteomeXchange identifier: PXD016303) were identified, of which 58 common differentially expressed proteins were significantly dysregulated in comparison between sham versus I/R and I/R versus DIO. The eight validated proteins including EPG5, STAT2, CPT1A, EIF2AK2, GGCT, HIKESHI, TNFAIP8, and EMC6 by quantitative polymerase chain reaction and western blotting consistently supported the TMT-based proteomic results, which were mainly associated with autophagy and inflammation response. Considering the anti-inflammatory characters of DIO, the biological functions of STAT2 and HIKESHI that are the probable direct anti-inflammatory targets were further investigated during the course of I/R treated with DIO. In addition, the combination of verified STAT2 and HIKESHI in peripheral blood samples from stroke patients resulted in the area under the curve value of 0.765 with P < 0.004 to distinguish stroke patients from healthy controls. Taken together, the current findings first mapped comprehensive proteomic changes after I/R was treated with DIO to better decipher the molecular mechanisms mainly based on the anti-inflammatory aspect underlying this therapeutic effect, providing a foundation for developing potentially therapeutic targets of anti-I/R of DIO and clinically prognostic biomarkers of stroke. |
| 源URL | [http://210.75.249.4/handle/363003/60396]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Xinxin,Wang, Xingbin,Khurm, Muhammad,et al. Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(3). |
| APA | Zhang, Xinxin.,Wang, Xingbin.,Khurm, Muhammad.,Zhan, Guanqun.,Zhang, Hui.,...&Guo, Zengjun.(2020).Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects.JOURNAL OF PROTEOME RESEARCH,19(3). |
| MLA | Zhang, Xinxin,et al."Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral lschemia Reperfusion Effects".JOURNAL OF PROTEOME RESEARCH 19.3(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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