Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots
文献类型：期刊论文
| 作者 | Zhu, Shuyun; Yan, Xiaolu; Qiu, Jiayi; Sun, Jing ; Zhao, Xian-En
|
| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
 |
| 出版日期 | 2020 |
| 卷号 | 225 |
| 关键词 | Graphene quantum dots Polydopamine Fluorescence resonance energy transfer Antioxidants Microdialysates |
| 英文摘要 | We describe a sensitive turn-on fluorescent assay for antioxidants by using fluorescence-tunable graphene quantum dots (GQDs). GQDs exhibited strong fluorescence without dopamine (DA). DA could self-polymerize to a thin polydopamine (PDA) film on the surface of GQDs under alkaline environment, resulting in the fluorescence quenching of GQDs via fluorescence resonance energy transfer (FRET). However, the self-polymerization of DA could be effectively inhibited in the presence of antioxidants including glutathione (GSH), ascorbic acid (AA), cysteine (Cys), and homocysteine (Hcys). Thus, the fluorescence of GQDs restored. The "turn-on" sensing of antioxidants could be achieved with high sensitivity. The detection limit for GSH, AA, Cys, and Hcys could be achieved as low as 2.4 nM, 1.5 nM, 4.2 nM, and 4.4 nM, respectively. Finally, the GQDs@PDA system was applied for monitoring cerebral antioxidants in rat brain microdialysates. This work promises new opportunities to evaluate antioxidant capacity in physiological and pathological fields. (C) 2019 Elsevier B.V. All rights reserved. |
| 源URL | [http://210.75.249.4/handle/363003/60441]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, Shuyun,Yan, Xiaolu,Qiu, Jiayi,et al. Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2020,225. |
| APA | Zhu, Shuyun,Yan, Xiaolu,Qiu, Jiayi,Sun, Jing,&Zhao, Xian-En.(2020).Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,225. |
| MLA | Zhu, Shuyun,et al."Turn-on fluorescent assay for antioxidants based on their inhibiting polymerization of dopamine on graphene quantum dots".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 225(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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