Transcriptome analysis identifies key genes involved in carotenoid biosynthesis in the flesh of red pummelo (Citrus maxima)
文献类型：期刊论文
| 作者 | Wang, Dongxia; Li, Shiming; Wei, Le; Li, Zongren; Liu, Baolong; Cao, Dong |
| 刊名 | BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
 |
| 出版日期 | 2020 |
| 卷号 | 34期号:1 |
| 关键词 | Pummelo red flesh carotenoid biosynthesis transcriptome analysis bHLH |
| 英文摘要 | Red-fleshed pummelo (Citrus grandis), a mutant of 'Guanxi', is an important economic plant rich in carotenoids. The transcriptome of 'Guanxi' pummelo was compared with red-fleshed pummelo, with the aim of identifying the key genes responsible for red flesh through RNA sequencing. In total, 44.11 and 47.71 Gb clean reads were respectively obtained after filtering in red and white flesh. Altogether 46,425 unigenes, with an average length of 1208 bp, were assembled. Compared with that of white flesh, 3722 genes were up-regulated, whereas 1421 genes appeared down-regulated in the red-fleshed pummelo. A GO analysis assigned 11,983 differentially expressed genes to 53 subcategories, and 3582, 4722 and 3679 differentially expressed genes were involved in biological processes, cellular components and molecular functions, respectively. The mevalonate pathway involved in terpenoid backbone biosynthesis was significantly different between 'Guanxi' pummelo and its red-fleshed mutant, and the differences were closely associated with carotenoid biosynthesis. The expression levels of the carotenoid biosynthetic pathway genes were different between 'Guanxi' pummelo and its red-fleshed mutant, with the upstream genes having higher expression levels in red-fleshed pummelo than in 'Guanxi' pummelo, whereas the downstream gene beta-CHX was downregulated. Two bHLH transcription factors (CL4778.Contig1 and CL4778.Contig2) were of the same branch as CubHLH1, regulating carotenoid biosynthesis inCitrus unshiuMarc., and Unigene CL4778.Contig2 had expression differences in two kinds of pummelo, which suggested CL4778.Contig2 should be a candidate gene controlling carotenoid accumulation in red pummelo. The study would be beneficial to identify the major gene regulating the red flesh trait. |
| 源URL | [http://210.75.249.4/handle/363003/60454]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Dongxia,Li, Shiming,Wei, Le,et al. Transcriptome analysis identifies key genes involved in carotenoid biosynthesis in the flesh of red pummelo (Citrus maxima)[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2020,34(1). |
| APA | Wang, Dongxia,Li, Shiming,Wei, Le,Li, Zongren,Liu, Baolong,&Cao, Dong.(2020).Transcriptome analysis identifies key genes involved in carotenoid biosynthesis in the flesh of red pummelo (Citrus maxima).BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,34(1). |
| MLA | Wang, Dongxia,et al."Transcriptome analysis identifies key genes involved in carotenoid biosynthesis in the flesh of red pummelo (Citrus maxima)".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 34.1(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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