Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat
文献类型：期刊论文
| 作者 | Zhao, Shuo; Xi, Xingyuan; Zong, Yuan; Li, Shiming; Li, Yun; Cao, Dong; Liu, Baolong |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2020 |
| 卷号 | 21期号:1 |
| 关键词 | wheat anthocyanin biosynthesis bHLH transcription factor ThMYC4E overexpression antioxidant activity |
| 英文摘要 | The basic helix-loop helix (bHLH) transcription factor has been inferred to play an important role in blue and purple grain traits in common wheat, but to date, its overexpression has not been reported. In this study, the bHLH transcription factor ThMYC4E, the candidate gene controlling the blue grain trait from Th. Ponticum, was transferred to the common wheat JW1. The positive transgenic lines displayed higher levels of purple anthocyanin pigments in their grains, leaves and glumes. Stripping the glumes (light treatment) caused white grains to become purple in transgenic lines. RNA-Seq and qRT-PCR analysis demonstrated that the transcript levels of structural genes associated with anthocyanin biosynthesis were higher in transgenic wheat than the wild-type (WT), which indicated that ThMYC4E activated anthocyanin biosynthesis in the transgenic lines. Correspondingly, the anthocyanin contents in grains, roots, stems, leaves and glumes of transgenic lines were higher than those in the WT. Metabolome analysis demonstrated that the anthocyanins were composed of cyanidin and delphinidin in the grains of the transgenic lines. Moreover, the transgenic lines showed higher antioxidant activity, in terms of scavenging DPPH radicals, in the ethanol extracts of their grains. The overexpression of ThMYC4E sheds light on the traits related to anthocyanin biosynthesis in common wheat and provide a new way to improve anthocyanin content. |
| 源URL | [http://210.75.249.4/handle/363003/60463]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Shuo,Xi, Xingyuan,Zong, Yuan,et al. Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(1). |
| APA | Zhao, Shuo.,Xi, Xingyuan.,Zong, Yuan.,Li, Shiming.,Li, Yun.,...&Liu, Baolong.(2020).Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(1). |
| MLA | Zhao, Shuo,et al."Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.1(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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