Determination of adenosine triphosphate based on the use of fluorescent terbium(III) organic frameworks and aptamer modified gold nanoparticles
文献类型：期刊论文
| 作者 | Sun, Chao; Zhao, Shiyu; Qu, Fei; Han, Wenli; You, Jinmao
|
| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2020 |
| 卷号 | 187期号:1 |
| 关键词 | Thiol-labeled aptamer Steric repulsion Electrostatic repulsion Unlabeled aptamer Inner filter effect Dynamic quenching Fluorescence resonance energy transfer Biosensing ATP-binding aptamer Aggregation |
| 英文摘要 | A thiol-labeled adenosine triphosphate (ATP) binding aptamer is covalently linked on the surface of gold nanoparticles (AuNPs). This warrants protection of the red AuNPs from aggregation in high salt condition. The dispersed AuNPs can quench the fluorescence of the Tb(III)-MOFs at 547 nm with the excitation wavelength of 290 nm. This is ascribed to the combined action of inner filter effect, dynamic quenching and fluorescence resonance energy transfer. If the aptamer binds ATP to form folded structures, the AuNPs aggregate in high salt medium and the green fluorescence of the Tb(III)-MOFs is recovered. This method shows good sensitivity and selectivity for ATP, and the linear range is from 0.5 to 10 mu M of ATP with the detection limitat of 0.32 mu M. It was applied to the determination of ATP in (spiked) human plasma with satisfactory recoveries (from 93.2% to 106.3%). Oppositely, when the unlabeled aptamer is used instead of thiol-labeled aptamer in this process, the ATP-aptamer complexes rather than unlabeled aptamer provide greater protection for AuNPs against salt-induced aggregation. It is found that when the aptamer covalently binds to AuNPs, the steric hindrance is dominant for the stabilization of AuNPs; for unlabeled aptamer, the electrostatic repulsion is responsible for their stability, irrespective of whether ATP is present or not. These two different forces lead to the aggregation or dispersion of AuNPs with addition of target in salt solution. |
| 源URL | [http://210.75.249.4/handle/363003/60470]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Chao,Zhao, Shiyu,Qu, Fei,et al. Determination of adenosine triphosphate based on the use of fluorescent terbium(III) organic frameworks and aptamer modified gold nanoparticles[J]. MICROCHIMICA ACTA,2020,187(1). |
| APA | Sun, Chao,Zhao, Shiyu,Qu, Fei,Han, Wenli,&You, Jinmao.(2020).Determination of adenosine triphosphate based on the use of fluorescent terbium(III) organic frameworks and aptamer modified gold nanoparticles.MICROCHIMICA ACTA,187(1). |
| MLA | Sun, Chao,et al."Determination of adenosine triphosphate based on the use of fluorescent terbium(III) organic frameworks and aptamer modified gold nanoparticles".MICROCHIMICA ACTA 187.1(2020). |
入库方式： OAI收割
来源：西北高原生物研究所
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